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DT elisa kit :: Human Dopachrome Tautomerase ELISA Kit

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Catalog # MBS008611
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
DT elisa kit
Product Name

Dopachrome Tautomerase (DT), ELISA Kit

Also Known As

Human Dopachrome Tautomerase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 13; NC_000013.10 (95091835..95131936, complement). Location: 13q32
OMIM
191275
3D Structure
ModBase 3D Structure for P40126
Species Reactivity
Specificity
No significant cross-reactivity or interference between Human DT and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DT concentrations in Human serum, plasma and other body fluids. Using Purified Human DT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DT and DT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forDTpurchase
MBS008611 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dopachrome Tautomerase (DT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DT. The ELISA analytical biochemical technique of the MBS008611 kit is based on DT antibody-DT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DT elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DT concentrations in Human serum, plasma and other body fluids. Using Purified Human DT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DT and DT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS008611. Click to request current manual
NCBI/Uniprot data below describe general gene information for DT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,145 Da
NCBI Official Full Name
dopachrome tautomerase, partial
NCBI Official Synonym Full Names
dopachrome tautomerase
NCBI Official Symbol
NCBI Official Synonym Symbols
TRP-2; TYRP2
  [Similar Products]
NCBI Protein Information
L-dopachrome tautomerase; DT; TRP2; dopachrome delta-isomerase; tyrosine-related protein 2; L-dopachrome Delta-isomerase; tyrosinase-related protein 2; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
UniProt Protein Name
L-dopachrome tautomerase
UniProt Synonym Protein Names
L-dopachrome Delta-isomerase; Tyrosinase-related protein 2
UniProt Gene Name
UniProt Synonym Gene Names
TYRP2; DCT; DT; TRP-2; TRP2  [Similar Products]
UniProt Entry Name
TYRP2_HUMAN
UniProt Comments for DT
DCT: Involved in regulating eumelanin and phaeomelanin levels. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell development/differentiation; Isomerase; Amino Acid Metabolism - tyrosine; EC 5.3.3.12

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: melanosome membrane; melanosome; integral to membrane; cytosol

Molecular Function: copper ion binding; dopachrome isomerase activity; oxidoreductase activity

Biological Process: neuroblast division in the ventricular zone; positive regulation of neuroblast proliferation; epidermis development; pigmentation during development; melanin biosynthetic process from tyrosine; cell development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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