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DT elisa kit :: Human DT ELISA Kit

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Catalog # MBS2509575
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

DT, ELISA Kit

Popular Item
Full Product Name

Human DT (Dopachrome Tautomerase) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
113810
3D Structure
ModBase 3D Structure for Q03001
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human DT. No significant cross-reactivity or interference between Human DT and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human DT and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DTpurchase
MBS2509575 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DT, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DT. The ELISA analytical biochemical technique of the MBS2509575 kit is based on DT antibody-DT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
DT elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DT in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human DT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human DT. You can calculate the concentration of Human DT in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of DT elisa kit
DT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2509575. Click to request current manual
NCBI/Uniprot data below describe general gene information for DT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
102,868 Da
NCBI Official Full Name
dystonin isoform 2
NCBI Official Synonym Full Names
dystonin
NCBI Official Symbol
NCBI Official Synonym Symbols
DT; BPA; DMH; BP240; BPAG1; EBSB2; HSAN6; MACF2; CATX15; CATX-15; D6S1101
  [Similar Products]
NCBI Protein Information
dystonin; bullous pemphigoid antigen 1; dystonia musculorum protein; hemidesmosomal plaque protein; trabeculin-beta
UniProt Protein Name
Dystonin
UniProt Synonym Protein Names
230 kDa bullous pemphigoid antigen; 230/240 kDa bullous pemphigoid antigen; Bullous pemphigoid antigen 1; BPA; Bullous pemphigoid antigen; Dystonia musculorum protein; Hemidesmosomal plaque protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BP230; BP240; BPAG1; DMH; DT; KIAA0728; BPA; Bullous pemphigoid antigen  [Similar Products]
UniProt Entry Name
DYST_HUMAN
NCBI Summary for DT
This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq, Mar 2010]
UniProt Comments for DT
BPAG1 iso3: a protein of the plakin family. Plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. Ten alternatively spliced human isoforms have been reported. BPAG1 has neuronal and muscle isoforms that consist of actin-binding and microtubule-binding domains at either end separated by a plakin domain and many spectrin repeats. Bpag1 isoforms with different N-termini may have differing roles. The plakin domain contains an apparent nuclear localization signal.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal; Microtubule-binding

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: intermediate filament cytoskeleton; endoplasmic reticulum membrane; focal adhesion; cytoplasmic membrane-bound vesicle; leading edge; integral to membrane; intermediate filament; nuclear envelope; cell cortex; cytosol; Z disc; actin cytoskeleton; microtubule cytoskeleton; hemidesmosome; axon; cytoplasm; basal plasma membrane; basement membrane; nucleus

Molecular Function: integrin binding; protein C-terminus binding; microtubule plus-end binding; protein binding; protein homodimerization activity; calcium ion binding; actin binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; hemidesmosome assembly; cell motility involved in cell locomotion; response to wounding; intermediate filament cytoskeleton organization and biogenesis; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; cell adhesion; maintenance of cell polarity; cell cycle arrest
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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