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EAAT1 elisa kit :: Rat Excitatory Amino Acid Transporter 1 ELISA Kit

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Catalog # MBS050004
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
EAAT1 elisa kit
Product Name

Excitatory Amino Acid Transporter 1 (EAAT1), ELISA Kit

Popular Item
Also Known As

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
496
OMIM
600111
3D Structure
ModBase 3D Structure for P43003
Species Reactivity
Specificity
No significant cross-reactivity or interference between Rat EAAT1 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EAAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEAAT1purchase
MBS050004 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EAAT1. The ELISA analytical biochemical technique of the MBS050004 kit is based on EAAT1 antibody-EAAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EAAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EAAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EAAT1 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS050004. Click to request current manual
NCBI/Uniprot data below describe general gene information for EAAT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,572 Da
NCBI Official Full Name
excitatory amino acid transporter 1 isoform 4
NCBI Official Synonym Full Names
solute carrier family 1 (glial high affinity glutamate transporter), member 3
NCBI Official Symbol
SLC1A3  [Similar Products]
NCBI Official Synonym Symbols
EA6; EAAT1; GLAST; GLAST1
  [Similar Products]
NCBI Protein Information
excitatory amino acid transporter 1; GLAST-1; solute carrier family 1 member 3; sodium-dependent glutamate/aspartate transporter 1
UniProt Protein Name
Excitatory amino acid transporter 1
UniProt Synonym Protein Names
Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3
UniProt Gene Name
SLC1A3  [Similar Products]
UniProt Synonym Gene Names
EAAT1; GLAST; GLAST1; GLAST-1  [Similar Products]
UniProt Entry Name
EAA1_HUMAN
NCBI Summary for EAAT1
This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
UniProt Comments for EAAT1
SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: cell surface; cell projection; membrane; cell soma; fibril; integral to membrane; plasma membrane

Molecular Function: glutamate binding; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; response to light stimulus; cranial nerve development; auditory behavior; glutamate biosynthetic process; positive regulation of synaptic transmission; response to antibiotic; L-glutamate import; synaptic transmission; sensory perception of sound; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neurotransmitter uptake; ion transport; neuromuscular process controlling balance; transmembrane transport

Disease: Episodic Ataxia, Type 6
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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