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EAAT2 elisa kit :: Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit

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Catalog # MBS2020019 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
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Typical Testing Data/Standard Curve
Product Name

Excitatory Amino Acid Transporter 2 (EAAT2), ELISA Kit

Popular Item
Full Product Name

Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit

Product Synonym Names
SLC1A2; EAAT2; GLT-1; Solute Carrier Family 1 Member 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600300
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Excitatory Amino Acid Transporter 2 (EAAT2).
No significant cross-reactivity or interference between Excitatory Amino Acid Transporter 2 (EAAT2) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EAAT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for EAAT2purchase
MBS2020019 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EAAT2. The ELISA analytical biochemical technique of the MBS2020019 kit is based on EAAT2 antibody-EAAT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EAAT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EAAT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EAAT2 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 2 (EAAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 2 (EAAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for EAAT2 elisa kit

Typical Testing Data/Standard Curve of EAAT2 elisa kit
EAAT2 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS2020019. Click to request current manual
NCBI/Uniprot data below describe general gene information for EAAT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,008 Da
NCBI Official Full Name
excitatory amino acid transporter 2 isoform 2
NCBI Official Synonym Full Names
solute carrier family 1 (glial high affinity glutamate transporter), member 2
NCBI Official Symbol
SLC1A2  [Similar Products]
NCBI Official Synonym Symbols
HBGT; EAAT2; GLT-1
  [Similar Products]
NCBI Protein Information
excitatory amino acid transporter 2
UniProt Protein Name
Excitatory amino acid transporter 2
UniProt Synonym Protein Names
Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2
UniProt Gene Name
SLC1A2  [Similar Products]
UniProt Synonym Gene Names
EAAT2; GLT1  [Similar Products]
UniProt Entry Name
EAA2_HUMAN
NCBI Summary for EAAT2
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]
UniProt Comments for EAAT2
GLT1: an integral membrane protein belonging to the sodium:dicarboxylate (SDF) symporter family. Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by co-transporting sodium.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p13-p12

Cellular Component: membrane; plasma membrane; integral to membrane; axolemma

Molecular Function: protein binding; glutamate:sodium symporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; synaptic transmission; positive regulation of glucose import; L-glutamate import; telencephalon development; adult behavior; multicellular organism growth; cellular response to extracellular stimulus; response to wounding; multicellular organismal aging; ion transport; response to amino acid stimulus; transmembrane transport; visual behavior
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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