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EAAT3 elisa kit :: Human Excitatory Amino Acid Transporter 3 ELISA Kit

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Catalog # MBS2533406
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Excitatory Amino Acid Transporter 3 (EAAT3), ELISA Kit

Popular Item
Full Product Name

Human EAAT3 (Excitatory Amino Acid Transporter 3) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
133550
Species Reactivity
Specificity
This kit recognizes natural and recombinantHumanEAAT3. No significant cross-reactivity or interference between HumanEAAT3 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
Min: 0.469ng/mL; Max: 50ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EAAT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for EAAT3purchase
MBS2533406 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EAAT3. The ELISA analytical biochemical technique of the MBS2533406 kit is based on EAAT3 antibody-EAAT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EAAT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EAAT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EAAT3 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanEAAT3concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EAAT3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EAAT3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EAAT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of EAAT3.You can calculate the concentration of EAAT3in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of EAAT3 elisa kit
EAAT3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2533406. Click to request current manual
NCBI/Uniprot data below describe general gene information for EAAT3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,100 Da
NCBI Official Full Name
excitatory amino acid transporter 3
NCBI Official Synonym Full Names
solute carrier family 1 member 1
NCBI Official Symbol
SLC1A1  [Similar Products]
NCBI Official Synonym Symbols
DCBXA; EAAC1; EAAT3; SCZD18
  [Similar Products]
NCBI Protein Information
excitatory amino acid transporter 3
UniProt Protein Name
Excitatory amino acid transporter 3
UniProt Synonym Protein Names
Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1
UniProt Gene Name
SLC1A1  [Similar Products]
UniProt Synonym Gene Names
EAAC1; EAAT3  [Similar Products]
UniProt Entry Name
EAA3_HUMAN
NCBI Summary for EAAT3
This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010]
UniProt Comments for EAAT3
SLC1A1: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: apical plasma membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: cysteine transmembrane transporter activity; glutamate binding; glutamate:sodium symporter activity; L-glutamate transmembrane transporter activity; protein binding; sodium:dicarboxylate symporter activity

Biological Process: cysteine transport; glutamate secretion; ion transport; L-glutamate import; neurotransmitter secretion; positive regulation of heart rate; protein homooligomerization; synaptic transmission; transmembrane transport

Disease: Schizophrenia 18
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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