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Ecm1 elisa kit :: Mouse Extracellular matrix protein 1 ELISA Kit

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Catalog # MBS2886761
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  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Ecm1 elisa kit
Product Name

Extracellular matrix protein 1 (Ecm1), ELISA Kit

Popular Item
Full Product Name

Mouse Extracellular matrix protein 1 ELISA Kit

Product Synonym Names
Extracellular matrix protein 1; Secretory component p85; Ecm1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2886761 Testing Data
3D Structure
ModBase 3D Structure for Q61508
Species Reactivity
Assay Type
Sandwich
Detection Range
0.312-20 ng/mL
Sensitivity
0.159 ng/mL
Intra-Assay CV
<=5.2%
Inter-Assay CV
<=7.7%
Recovery
85%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Ecm1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEcm1purchase
MBS2886761 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Extracellular matrix protein 1 (Ecm1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ecm1. The ELISA analytical biochemical technique of the MBS2886761 kit is based on Ecm1 antibody-Ecm1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ecm1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ecm1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Ecm1 elisa kit
Intended Uses||This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS2886761. Click to request current manual
NCBI/Uniprot data below describe general gene information for Ecm1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
48,357 Da
NCBI Official Full Name
extracellular matrix protein 1 isoform 1
UniProt Protein Name
Extracellular matrix protein 1
UniProt Synonym Protein Names
Secretory component p85
UniProt Gene Name
UniProt Entry Name
ECM1_MOUSE
UniProt Comments for Ecm1
ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: enzyme binding; interleukin-2 receptor binding; laminin binding; protease binding; protein C-terminus binding; signal transducer activity

Biological Process: angiogenesis; biomineral formation; inflammatory response; negative regulation of bone mineralization; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of peptidase activity; ossification; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of bone mineralization; regulation of T-helper 2 type immune response; regulation of transcription from RNA polymerase II promoter; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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