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ECP elisa kit :: Mouse eosinophil cationic protein, ECP ELISA Kit

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Catalog # MBS162764
Unit / Price
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  48-Strip-Wells  /  $280 +1 FREE 8GB USB
  96-Strip-Wells  /  $445 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,830 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,580 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

eosinophil cationic protein, ECP, ELISA Kit

Popular Item
Full Product Name

Mouse eosinophil cationic protein, ECP ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P12724
Species Reactivity
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
20ng/L - 6000ng/L
Sensitivity
10.46ng/L
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ECP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ECPpurchase
MBS162764 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eosinophil cationic protein, ECP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ECP. The ELISA analytical biochemical technique of the MBS162764 kit is based on ECP antibody-ECP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ECP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ECP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ECP elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Eosinophil Cationic Protein (also known as ECP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ECP antibody. ECP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ECP Antibody is added and binds to ECP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ECP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ECP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Typical Testing Data/Standard Curve (for reference only) of ECP elisa kit
ECP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS162764. Click to request current manual
NCBI/Uniprot data below describe general gene information for ECP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,385 Da
NCBI Official Full Name
eosinophil cationic protein
NCBI Official Synonym Full Names
ribonuclease A family member 3
NCBI Official Symbol
RNASE3  [Similar Products]
NCBI Official Synonym Symbols
ECP; RAF1; RNS3
  [Similar Products]
NCBI Protein Information
eosinophil cationic protein
UniProt Protein Name
Eosinophil cationic protein
UniProt Synonym Protein Names
Ribonuclease 3; RNase 3
UniProt Gene Name
RNASE3  [Similar Products]
UniProt Synonym Gene Names
ECP; RNS3; ECP; RNase 3  [Similar Products]
NCBI Summary for ECP
The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
UniProt Comments for ECP
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with ECP elisa kit
Organs/Tissues associated with ECP elisa kit
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