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EDN1 elisa kit :: Porcine Endothelin 1 (EDN1) ELISA Kit

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Catalog # MBS2700168 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells  /  $230 +1 FREE 8GB USB
  48-Strip-Wells  /  $380 +1 FREE 8GB USB
  96-Strip-Wells  /  $505 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,115 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,930 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Endothelin 1 (EDN1), ELISA Kit

Popular Item
Also Known As

Pig Endothelin 1 (EDN1) ELISA Kit

Product Synonym Names
ET1; PPET1; Preproendothelin-1; Big endothelin-1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
212
OMIM
Y00749 mRNA
3D Structure
ModBase 3D Structure for P05305
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1).
No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
Detection Range
12.35-1000pg/mL
Sensitivity
< 4.74pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EDN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEDN1purchase
MBS2700168 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endothelin 1 (EDN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EDN1. The ELISA analytical biochemical technique of the MBS2700168 kit is based on EDN1 antibody-EDN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EDN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EDN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EDN1 elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin 1 (EDN1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin 1 (EDN1) and unlabeled Endothelin 1 (EDN1) (Standards or samples) with the pre-coated antibody specific to Endothelin 1 (EDN1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample.

Typical Testing Data/Standard Curve (for reference only) of EDN1 elisa kit
EDN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2700168. Click to request current manual
NCBI/Uniprot data below describe general gene information for EDN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,425 Da
NCBI Official Full Name
Endothelin-1
NCBI Official Synonym Full Names
endothelin 1
NCBI Official Symbol
NCBI Official Synonym Symbols
ET1; QME; PPET1; ARCND3; HDLCQ7
  [Similar Products]
NCBI Protein Information
endothelin-1
UniProt Protein Name
Endothelin-1
UniProt Synonym Protein Names
Preproendothelin-1; PPET1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PPET1; ET-1  [Similar Products]
NCBI Summary for EDN1
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Comments for EDN1
Endothelins are endothelium-derived vasoconstrictor peptides.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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