• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Efabp5 elisa kit :: Rat Epidermal fatty acid binding protein 5 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS753790
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Epidermal fatty acid binding protein 5 (Efabp5), ELISA Kit

Popular Item
Also Known As

Rat Epidermal fatty acid binding protein 5 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 2; NC_005101.3 (113735159..113738819, complement). Location: chromosome: 2
3D Structure
ModBase 3D Structure for P55053
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of FABP5. No significant cross-reactivity or interference between FABP5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FABP5 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Sensitivity
1.0 pg/mL.
Intended Uses
This FABP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FABP5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Efabp5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEfabp5purchase
MBS753790 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epidermal fatty acid binding protein 5 (Efabp5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Efabp5. The ELISA analytical biochemical technique of the MBS753790 kit is based on Efabp5 antibody-Efabp5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Efabp5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Efabp5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Efabp5 elisa kit
Principle of the assay: FABP5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FABP5. Standards or samples are then added to the microtiter plate wells and FABP5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FABP5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FABP5 are added to each well to "sandwich" the FABP5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FABP5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Efabp5 elisa kit

Typical Testing Data/Standard Curve (for reference only) of Efabp5 elisa kit
Efabp5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS753790. Click to request current manual
NCBI/Uniprot data below describe general gene information for Efabp5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,059 Da
NCBI Official Full Name
epidermal fatty acid binding protein 5
NCBI Official Synonym Full Names
fatty acid binding protein 5, epidermal
NCBI Official Symbol
Fabp5  [Similar Products]
NCBI Official Synonym Symbols
DA11; C-FABP; E-FABP
  [Similar Products]
NCBI Protein Information
fatty acid-binding protein, epidermal; fatty acid-binding protein 5; cutaneous fatty acid-binding protein; epidermal-type fatty acid-binding protein
UniProt Protein Name
Fatty acid-binding protein, epidermal
UniProt Synonym Protein Names
Cutaneous fatty acid-binding protein; C-FABP; DA11; Epidermal-type fatty acid-binding protein; E-FABP; Fatty acid-binding protein 5
UniProt Gene Name
Fabp5  [Similar Products]
UniProt Synonym Gene Names
C-FABP; E-FABP  [Similar Products]
UniProt Entry Name
FABP5_RAT
NCBI Summary for Efabp5
binds long chain fatty acids [RGD, Feb 2006]
UniProt Comments for Efabp5
FABP5: High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: cytoplasm

Molecular Function: transporter activity; fatty acid binding

Biological Process: transport; phosphatidylcholine biosynthetic process; response to wounding; glucose metabolic process; glucose transport; lipid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with Efabp5 elisa kit
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000