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EFEMP1 elisa kit :: Human Fibulin-3/EFEMP1 ELISA Kit

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Catalog # MBS178533
Unit / Price
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  96-Strip-Wells  /  $425 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Fibulin-3/EFEMP1, ELISA Kit

Popular Item
Full Product Name

Human Fibulin-3/EFEMP1 PicoKine ELISA Kit

Product Synonym Names
DHRD; DRAD; EFEMP 1; EFEMP1; FBLN 3; FBLN3; FBNL; FIBL3; FIBL-3; FIBL 3; FIBULIN 3; FIBULIN-3; FIBULIN3; MLVT; MTLV; S1 5; T16 protein; Q12805; EGF-containing fibulin-like extracellular matrix protein 1; EGF containing fibulin-like extracellular matrix protein 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
126600
3D Structure
ModBase 3D Structure for Q12805
Species Reactivity
Specificity
Natural and recombinant Human Fibulin-3/EFEMP1
Samples
Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
312pg/ml-20000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EFEMP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEFEMP1purchase
MBS178533 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibulin-3/EFEMP1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EFEMP1. The ELISA analytical biochemical technique of the MBS178533 kit is based on EFEMP1 antibody-EFEMP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EFEMP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EFEMP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EFEMP1 elisa kit
Principle of the Assay: Human Fibulin-3/EFEMP1 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A specific for Fibulin-3/EFEMP1 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: Q18-F493) and test samples are added to the wells, a biotinylated detection specific for Fibulin-3/EFEMP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Fibulin-3/EFEMP1 amount of sample captured in plate.

Background/Introduction: EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.

Typical Testing Data/Standard Curve (for reference only) of EFEMP1 elisa kit
Human Fibulin-3/EFEMP1 PicoKine ELISA Kit standard curve
EFEMP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS178533. Click to request current manual
NCBI/Uniprot data below describe general gene information for EFEMP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,195 Da
NCBI Official Full Name
EGF-containing fibulin-like extracellular matrix protein 1
NCBI Official Synonym Full Names
EGF containing fibulin like extracellular matrix protein 1
NCBI Official Symbol
EFEMP1  [Similar Products]
NCBI Official Synonym Symbols
DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3
  [Similar Products]
NCBI Protein Information
EGF-containing fibulin-like extracellular matrix protein 1
UniProt Protein Name
EGF-containing fibulin-like extracellular matrix protein 1
UniProt Synonym Protein Names
Extracellular protein S1-5; Fibrillin-like protein; Fibulin-3; FIBL-3
UniProt Gene Name
EFEMP1  [Similar Products]
UniProt Synonym Gene Names
FBLN3; FBNL; FIBL-3  [Similar Products]
UniProt Entry Name
FBLN3_HUMAN
NCBI Summary for EFEMP1
This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]
UniProt Comments for EFEMP1
EFEMP1: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD); also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: epidermal growth factor receptor activity; epidermal growth factor receptor binding; protein binding

Biological Process: camera-type eye development; embryonic eye morphogenesis; epidermal growth factor receptor signaling pathway; negative regulation of chondrocyte differentiation; peptidyl-tyrosine phosphorylation; post-embryonic eye morphogenesis; regulation of transcription, DNA-dependent; visual perception

Disease: Doyne Honeycomb Retinal Dystrophy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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