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EGF recombinant protein :: Epidermal Growth Factor Recombinant Protein

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Catalog # MBS142209
Unit / Price
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  0.1 mg  /  $140 +1 FREE 8GB USB
  0.5 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $305 +1 FREE 8GB USB
EGF recombinant protein
Product Name

Epidermal Growth Factor (EGF), Recombinant Protein

Popular Item
Full Product Name

Recombinant Human Epidermal Growth Factor, Pichia

Product Synonym Names
EGF Human, Pichia; Epidermal Growth Factor Human Recombinant, Pichia; Urogastrone; URG; EGF; EGF Pichia
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-As p-Ser-Glu, which agrees with the sequence of native EGF human.N-te rminal methionine has been completely removed enzymatica lly
3D Structure
ModBase 3D Structure for Q6QBS2
Host
Pichia Pastoris
Purity/Purification
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
Each lyophilized mg contains 0.15M NaCl, 0.025M sodium bicarbonate, pH-7.5.
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10,000,000Units/mg.
Preparation and Storage
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution EGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Other Notes
Small volumes of EGF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EGF recombinant protein
Description: Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa.The EGF is purified by proprietary chromatographic techniques.

Introduction: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
6,222 Da
NCBI Official Full Name
Epidermal growth factor
UniProt Protein Name
Epidermal growth factor
UniProt Synonym Protein Names
Epidermal growth factor
Protein Family
UniProt Gene Name
UniProt Entry Name
Q6QBS2_HUMAN
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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