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EGFLAM elisa kit :: Human Pikachurin ELISA Kit

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Catalog # MBS760605
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Pikachurin (EGFLAM), ELISA Kit

Popular Item
Full Product Name

Human Pikachurin ELISA Kit

Product Synonym Names
EGFLAM/Agrin-like protein/EGF-like; fibronectin type-III and laminin G-like domain-containing protein/AGRINL/AGRNL
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of EGFLAM. No significant cross-reactivity or interference between EGFLAM and analogues was observed.
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.312-20ng/ml
Sensitivity
0.188ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGFLAM were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGFLAM were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EGFLAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for EGFLAMpurchase
MBS760605 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pikachurin (EGFLAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EGFLAM. The ELISA analytical biochemical technique of the MBS760605 kit is based on EGFLAM antibody-EGFLAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EGFLAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EGFLAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EGFLAM elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- EGFLAM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- EGFLAM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EGFLAM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EGFLAM can be calculated.

Typical Testing Data/Standard Curve (for reference only) of EGFLAM elisa kit
EGFLAM elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for EGFLAM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,588 Da
NCBI Official Full Name
pikachurin isoform 5
NCBI Official Synonym Full Names
EGF like, fibronectin type III and laminin G domains
NCBI Official Symbol
EGFLAM  [Similar Products]
NCBI Official Synonym Symbols
PIKA; AGRNL; AGRINL
  [Similar Products]
NCBI Protein Information
pikachurin
UniProt Protein Name
Pikachurin
UniProt Synonym Protein Names
Agrin-like protein; EGF-like, fibronectin type-III and laminin G-like domain-containing protein
Protein Family
UniProt Gene Name
EGFLAM  [Similar Products]
UniProt Synonym Gene Names
AGRINL; AGRNL  [Similar Products]
UniProt Entry Name
EGFLA_HUMAN
UniProt Comments for EGFLAM
EGFLAM: Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13.2-p13.1

Cellular Component: basement membrane; cell junction; synapse

Molecular Function: glycosaminoglycan binding

Biological Process: extracellular matrix organization and biogenesis; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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