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EGFP recombinant protein

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Catalog # MBS8506782
Unit / Price
  0.1 mg  /  $180 +1 FREE 8GB USB
  0.2 mg  /  $235 +1 FREE 8GB USB
  0.5 mg  /  $320 +1 FREE 8GB USB
Fluorescence
Product Name

EGFP, Recombinant Protein

Popular Item
Full Product Name

EGFP-rProtein A

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
E. coli
Specificity
Under optimal conditions, 1 mg EGFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of EGFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
Purity/Purification
>98% by SDS-PAGE and HPLC
Form/Format
Liquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative.
Endotoxin
< 0.05 EU/mg
IgG Binding
>95%
Bioburden
No organisms detected
Preparation and Storage
Store at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.
Other Notes
Small volumes of EGFP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EGFP recombinant protein
The unique, high quality recombinant EGFP-rProtein A are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). EGFP-rProtein A bind to most mammalian immunoglobulins in their Fc binding domains and therefore are ideal for immunofluorescence staining. The genetically engineered protein was expressed and purified from transformed E. coli containing only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these proteins to ensure the maximum specific IgG binding and maximal fluorescence.
Application Notes for EGFP recombinant protein
The protein is suitable as control reagent for EGFP expression studies or as labeling reagent. Applications include: Flow cytometers, fluorescence microscopy, and microinjection, antibody purification, etc. at 1:100 dilution. Maximum absorption wavelength at 488 nm and emission wavelength at 507 nm.

Fluorescence of EGFP recombinant protein
293T cells were stained with mouse anti-beta-tubulin antibody and detected with EGFP-rProtein A (Green).
EGFP recombinant protein Fluorescence image
NCBI/Uniprot data below describe general gene information for EGFP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
eGFP
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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