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EIF2A elisa kit :: Rat Eukaryotic Translation Initiation Factor 2A ELISA Kit

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Catalog # MBS751823
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Eukaryotic Translation Initiation Factor 2A (EIF2A), ELISA Kit

Popular Item
Also Known As

Rat Eukaryotic Translation Initiation Factor 2A ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
609234
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of EIF-2A. No significant cross-reactivity or interference between EIF-2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EIF-2A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/ml
Intended Uses
This EIF-2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EIF-2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EIF2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEIF2Apurchase
MBS751823 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eukaryotic Translation Initiation Factor 2A (EIF2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EIF2A. The ELISA analytical biochemical technique of the MBS751823 kit is based on EIF2A antibody-EIF2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EIF2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EIF2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EIF2A elisa kit

Principle of the assay: EIF-2A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EIF-2A. Standards or samples are then added to the microtiter plate wells and EIF-2A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EIF-2A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for EIF-2A are added to each well to "sandwich" the EIF-2A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EIF-2A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EIF-2A concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of EIF2A elisa kit
EIF2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS751823. Click to request current manual
NCBI/Uniprot data below describe general gene information for EIF2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,339 Da
NCBI Official Full Name
eukaryotic translation initiation factor 2A
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2A, 65kDa
NCBI Official Symbol
EIF2A  [Similar Products]
NCBI Official Synonym Symbols
CDA02; EIF-2A; MST089; MSTP004; MSTP089
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 2A; 65 kDa eukaryotic translation initiation factor 2A
UniProt Protein Name
Eukaryotic translation initiation factor 2A
UniProt Synonym Protein Names
65 kDa eukaryotic translation initiation factor 2ACleaved into the following chain:Eukaryotic translation initiation factor 2A, N-terminally processed
UniProt Gene Name
EIF2A  [Similar Products]
UniProt Synonym Gene Names
CDA02; MSTP004; MSTP089; eIF-2A  [Similar Products]
UniProt Entry Name
EIF2A_HUMAN
NCBI Summary for EIF2A
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM, Mar 2008]
UniProt Comments for EIF2A
EIF2A: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Belongs to the WD repeat EIF2A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: extracellular space; cytoplasm; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding; tRNA binding

Biological Process: regulation of translation; positive regulation of signal transduction; ribosome assembly; translational initiation; SREBP-mediated signaling pathway; protein amino acid phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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