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eIF2alpha elisa kit

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Catalog # MBS9501438
Unit / Price
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  2x96 Wells  /  $535 +1 FREE 8GB USB
eIF2alpha elisa kit
Product Name

eIF2alpha, ELISA Kit

Popular Item
Full Product Name

Phospho-eIF2alpha (Ser51) Colorimetric Cell-Based ELISA Kit

Product Synonym Names
eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2S1; Eukaryotic translation initiation factor 2 alpha subunit; Eukaryotic translation initiation factor 2 subunit 1; IF2A
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
J02645 mRNA
3D Structure
ModBase 3D Structure for P05198
Species Reactivity
Form/Format
Two 96-Well Plates
Samples
Researchers plate their cell line of choice
Assay Type
Phospho Cell-Based ELISA
Sensitivity
>=5000 cells/well
Reactivity Note
Human: S52, Mouse: S52, Rat: S52
Detection Method
Colorimetric 450 nm
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of eIF2alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for eIF2alphapurchase
MBS9501438 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eIF2alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing eIF2alpha. The ELISA analytical biochemical technique of the MBS9501438 kit is based on eIF2alpha antibody-eIF2alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect eIF2alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, eIF2alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for eIF2alpha elisa kit
Sample Manual Insert of MBS9501438. Click to request current manual
NCBI/Uniprot data below describe general gene information for eIF2alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
36112
NCBI Official Full Name
eukaryotic translation initiation factor 2 subunit 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 subunit alpha
NCBI Official Symbol
EIF2S1  [Similar Products]
NCBI Official Synonym Symbols
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1
UniProt Protein Name
Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
UniProt Gene Name
EIF2S1  [Similar Products]
UniProt Synonym Gene Names
EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha  [Similar Products]
NCBI Summary for eIF2alpha
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
UniProt Comments for eIF2alpha
eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: cytosol; eukaryotic translation initiation factor 2 complex; eukaryotic translation initiation factor 2B complex; extracellular exosome; membrane; nucleus; polysome; ribosome; stress granule; translation initiation ternary complex

Molecular Function: protein binding; ribosome binding; RNA binding; translation initiation factor activity

Biological Process: aging; cellular response to amino acid starvation; cellular response to heat; cellular response to UV; negative regulation of guanyl-nucleotide exchange factor activity; negative regulation of translational initiation in response to stress; PERK-mediated unfolded protein response; positive regulation of neuron death; positive regulation of type B pancreatic cell apoptotic process; protein autophosphorylation; response to manganese-induced endoplasmic reticulum stress; translational initiation; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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