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EIF2C2 elisa kit :: Mouse eukaryotic translation initiation factor 2C, 2 ELISA Kit

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Catalog # MBS911752
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

eukaryotic translation initiation factor 2C, 2 (EIF2C2), ELISA Kit

Popular Item
Full Product Name

Mouse Protein argonaute-2, EIF2C2 ELISA Kit

Product Synonym Names
Mouse Protein argonaute-2 (EIF2C2) ELISA kit; AGO2; MGC3183; Q10; argonaute 2; eukaryotic translation initiation factor 2C; 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS911752 Testing Data
3D Structure
ModBase 3D Structure for Q8CJG0
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse EIF2C2. No significant cross-reactivity or interference between mouse EIF2C2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml.
Sensitivity
The minimum detectable dose of mouse EIF2C2 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EIF2C2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for EIF2C2purchase
MBS911752 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eukaryotic translation initiation factor 2C, 2 (EIF2C2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EIF2C2. The ELISA analytical biochemical technique of the MBS911752 kit is based on EIF2C2 antibody-EIF2C2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EIF2C2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EIF2C2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EIF2C2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EIF2C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF2C2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF2C2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF2C2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of EIF2C2 elisa kit
EIF2C2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS911752. Click to request current manual
NCBI/Uniprot data below describe general gene information for EIF2C2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
97,304 Da
NCBI Official Full Name
protein argonaute-2
NCBI Official Synonym Full Names
argonaute RISC catalytic subunit 2
NCBI Official Symbol
NCBI Official Synonym Symbols
Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493
  [Similar Products]
NCBI Protein Information
protein argonaute-2
UniProt Protein Name
Protein argonaute-2
UniProt Synonym Protein Names
Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; Piwi/argonaute family protein meIF2C2; Protein slicer
UniProt Gene Name
UniProt Synonym Gene Names
Eif2c2; Kiaa4215; Argonaute2; mAgo2; eIF-2C 2; eIF2C 2  [Similar Products]
UniProt Entry Name
AGO2_MOUSE
UniProt Comments for EIF2C2
AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA processing; RNA-binding; EC 3.1.26.n2

Cellular Component: polysome; mRNA cap complex; membrane; cytoplasm; dendrite; nucleus; cytosol; ribonucleoprotein complex

Molecular Function: mRNA binding; endoribonuclease activity; protein binding; siRNA binding; nuclease activity; miRNA binding; nucleic acid binding; hydrolase activity; RNA binding; endonuclease activity; metal ion binding; RNA 7-methylguanosine cap binding

Biological Process: regulation of translation; negative regulation of translational initiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; pre-microRNA processing; multicellular organismal development; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; RNA-mediated gene silencing; cell differentiation; post-embryonic development; mRNA cleavage
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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