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anti-eIF4E antibody :: eIF4E Antibody

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Catalog # MBS857179
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
  0.05 mg  /  $225 +1 FREE 8GB USB
Product Name

eIF4E, Antibody

Popular Item
Also Known As

eIF4E (Phospho-Ser209) Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
237
OMIM
133440
3D Structure
ModBase 3D Structure for P06730
Species Reactivity
Human, Mouse, Rat
Specificity
elF4E (phospho-Ser209) antibody detects endogenous levels of elF4E only when phosphorylated at serine 209.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-eIF4E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-eIF4E antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-eIF4E antibody
WB: 1:500~1:1000
IHC: 1:50~1:100

Immunohistochemistry (IHC) of anti-eIF4E antibody
P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E (phospho-Ser209) antibody.
anti-eIF4E antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-eIF4E antibody
Western blot analysis of extract from NIH/3T3 cells untreated or treated with 10% serum (15min), using elF4E (Ab-209) antibody and elF4E (phospho-Ser209) antibody.
anti-eIF4E antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for eIF4E. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,260 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E isoform 3
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E
NCBI Official Symbol
EIF4E  [Similar Products]
NCBI Official Synonym Symbols
CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 4E
UniProt Protein Name
Eukaryotic translation initiation factor 4E
UniProt Synonym Protein Names
eIF-4F 25 kDa subunit; mRNA cap-binding protein
UniProt Gene Name
EIF4E  [Similar Products]
UniProt Synonym Gene Names
EIF4EL1; EIF4F; eIF-4E; eIF4E  [Similar Products]
NCBI Summary for eIF4E
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Comments for eIF4E
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Product References and Citations for anti-eIF4E antibody
Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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