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EIF4E elisa kit :: Rat Eukaryotic Translation Initiation Factor 4E (EIF4E) ELISA Kit

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Catalog # MBS9352700
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EIF4E elisa kit
Product Name

Eukaryotic Translation Initiation Factor 4E (EIF4E), ELISA Kit

Popular Item
Full Product Name

Rat Eukaryotic Translation Initiation Factor 4E (EIF4E) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/ml - 2000 pg/ml
Sensitivity
10 pg/ml
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EIF4E elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for EIF4Epurchase
MBS9352700 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eukaryotic Translation Initiation Factor 4E (EIF4E) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EIF4E. The ELISA analytical biochemical technique of the MBS9352700 kit is based on EIF4E antibody-EIF4E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EIF4E antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EIF4E. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EIF4E elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of EIF4E (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9352700. Click to request current manual
NCBI/Uniprot data below describe general gene information for EIF4E. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,053 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E
NCBI Official Symbol
Eif4e  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 4E
UniProt Protein Name
Eukaryotic translation initiation factor 4E
UniProt Synonym Protein Names
eIF-4F 25 kDa subunit; mRNA cap-binding protein
UniProt Gene Name
Eif4e  [Similar Products]
UniProt Synonym Gene Names
eIF-4E; eIF4E  [Similar Products]
UniProt Entry Name
IF4E_RAT
NCBI Summary for EIF4E
binds to the mRNA 7-methylguanosine cap and mediates mRNA binding to the 40S ribosome in the rate limiting step in translational initiation [RGD, Feb 2006]
UniProt Comments for EIF4E
EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex.

Protein type: Translation initiation; Translation

Cellular Component: cytoplasm; eukaryotic translation initiation factor 4F complex; mRNA cap complex; perinuclear region of cytoplasm; protein complex; stress granule

Molecular Function: enzyme binding; eukaryotic initiation factor 4G binding; protein binding; translation initiation factor activity

Biological Process: behavioral fear response; G1/S transition of mitotic cell cycle; lung development; negative regulation of neuron differentiation; negative regulation of translation; positive regulation of mitotic cell cycle; regulation of translation; stem cell maintenance; translational initiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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