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ELANE protease :: Neutrophil Elastase, Lyophilized Protease

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Catalog # MBS135624
Unit / Price
  0.5 mg  /  $645 +1 FREE 8GB USB
  1 mg  /  $1,110 +1 FREE 8GB USB
ELANE protease
Product Name

Neutrophil Elastase, Lyophilized (ELANE), Protease

Popular Item
Also Known As

Human Neutrophil Elastase, Lyophilized

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
267
Chromosome Location
Chromosome: 19; NC_000019.9 (852291..856246). Location: 19p13.3
OMIM
130130
3D Structure
ModBase 3D Structure for P08246
Purity/Purification
>95% by SDS-PAGE analysis
Form/Format
Lyophilized powder
Concentration
lyophilized (lot specific)
Source
Human neutrophil
Specific Activity
875 U/mg on Suc-Ala-Ala-Ala-pNA
Buffer
Salt free
Extinction Coefficient
0.985
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from delivery
Other Notes
Small volumes of ELANE protease vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ELANE protease
Chromatographically purified from leucocytes of purulent human sputum and lyophilized. The lyophilized, salt-free, soluble powder is stable for over a year when stored at 5 C. The protein is greater than 95percent pure by SDS-PAGE and is free of cathepsin G, MPO and lysozyme. 800-900 units per mg of protein on the substrate Suc-Ala-Ala-Ala-pNA. 15000-19000 units per mg protein on the substrate MeO-Suc-Ala-Ala-Pro-Val-pNA. One unit will hydrolyze 1 nanomole of substrate per minute at pH 7.5 and 25 C. The following diluents and concentrations are recommended: 1) 1 mg/ml in 0.05 M Na Acetate pH 5.0 containing 0.1 M NaCl. Stable 7 days at 5 C (in ice). 2) 10 mg/ml in 50percent glycerol 50percent 0.02 M Na Acetate pH 5. Stable at least 60 days at -20 C with 20 cycles between -20 C and 5 C. 3) May be dissolved at 10 mg/ml in 2 mM HoAc and re-lyophilized in smaller quantities. Stability is better in acidic (pH 5.0) than basic buffers. Inactivation may occur at pH 8.0 due to autolysis and/or proteolysis by trypsin such as bovine pancreatic trypsin. Proteolysis can be inhibited by benzamidine. Autolysis and proteolysis can be inhibited by elastatinal. However, elastatinal is also an inhibitor of elastase. Low pH protects the elastase from both autolysis and proteolysis.
NCBI/Uniprot data below describe general gene information for ELANE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,500
NCBI Official Full Name
neutrophil elastase preproprotein
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
ELANE  [Similar Products]
NCBI Official Synonym Symbols
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
  [Similar Products]
NCBI Protein Information
neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
UniProt Protein Name
Neutrophil elastase
UniProt Synonym Protein Names
Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
Protein Family
UniProt Gene Name
ELANE  [Similar Products]
UniProt Synonym Gene Names
ELA2; HLE  [Similar Products]
UniProt Entry Name
ELNE_HUMAN
NCBI Summary for ELANE
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]
UniProt Comments for ELANE
ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Motility/polarity/chemotaxis; EC 3.4.21.37; Cell cycle regulation; Protease; Cell surface

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; transcriptional repressor complex; cytoplasm; extracellular region; secretory granule

Molecular Function: heparin binding; peptidase activity; protein binding; protease binding; serine-type endopeptidase activity; cytokine binding; endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of immune response; positive regulation of smooth muscle cell proliferation; negative regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 biosynthetic process; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; negative regulation of chemotaxis; proteolysis; phagocytosis; cellular calcium ion homeostasis; extracellular matrix disassembly; collagen catabolic process; positive regulation of MAP kinase activity; negative regulation of inflammatory response; defense response to bacterium; protein catabolic process; response to yeast; leukocyte migration; negative regulation of chemokine biosynthetic process; acute inflammatory response to antigenic stimulus; response to UV

Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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