View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List 96-Well Ras Activation ELISA Kit Catalog # MBS168173 Unit / Price 96 Assays / $1,090 +1 FREE 8GB USB 5x96 Assays / $5,040 +6 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name 96-Well Ras Activation, ELISA Kit Popular Item Full Product Name 96-Well Ras Activation ELISA Kit (Chemiluminescent) Product Synonym Names 96-Well Ras Activation ELISA Kit (Chemiluminescent) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Preparation and Storage Upon receipt, aliquot and store Raf-1 RBD at -80 degree C and avoid freeze/thaw. Aliquot and store the Anti-pan-Ras Antibody, Secondary Antibody, GTPgammaS, and GDP components at -20 degree C and avoid freeze/thaw. Store all other components at 4 degree C until their expiration dates. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of 96-Well Ras Activation elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for 96-Well Ras Activation purchase MBS168173 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 96-Well Ras Activation, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 96-Well Ras Activation. The ELISA analytical biochemical technique of the MBS168173 kit is based on 96-Well Ras Activation antibody-96-Well Ras Activation antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 96-Well Ras Activation antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 96-Well Ras Activation. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for 96-Well Ras Activation elisa kit Background/Introduction: Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Ras, a 21 kDa protein, regulates a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, Ras regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf-1 to control downstream signaling cascades. The most notable members of the Ras subfamily are H-Ras, N-Ras and K-Ras, mainly for being implicated in many types of cancer. 96-well Ras Activation ELISA Kit utilizes plate-bound, Raf-1 RBD to selectively isolate and pull-down the active forms of Ras (H-, K-, and N-Ras isoforms from human, mouse and rat) from purified samples or endogenous lysates. Subsequently, the captured GTP-Ras is detected by an Anti-pan-Ras Antibody and HRP conjugated secondary antibody. Finally, following the addition of Chemiluminescent Reagent, the reaction is then measured in a plate luminometer. 96-well Ras Activation ELISA Kit provides a simple and fast tool to monitor the activation of Ras. Each kit provides sufficient reagents to perform up to 96 assays. Product Categories/Family for 96-Well Ras Activation elisa kit Cell Signaling and Protein Biology; G Protein Signaling/Small GTPase Assays and Reagents; Small GTPase Research Tools; Small GTPase Activation ELISA Kits; Ras Activation ELISA; Chemiluminescent Testing Data of 96-Well Ras Activation elisa kit Testing Data #2 of 96-Well Ras Activation elisa kit TOP Sample Manual Insert of MBS168173. Product References and Citations for 96-Well Ras Activation elisa kit 1. Bar-Sagi D., and Hall A. (2000) Cell 103: 227-38.2. de Rooij J., and Bos J. L. (1997) Oncogene 14: 623-5. 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