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C3 ELISA Kit

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Catalog # MBS564013
Unit / Price
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  1x96 Wells  /  $405 +1 FREE 8GB USB
C3 elisa kit
Product Name

C3, ELISA Kit

Full Product Name

Dog C3 ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Host
Dog
Samples
Immunoperoxidase Assay for Determination of C3 in Dog Samples
Assay Type
Sandwich
Intended Uses
The Complement factor 3 (C3) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C3 in biological samples of dogs.
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of C3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forC3purchase
MBS564013 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dog C3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C3. The ELISA analytical biochemical technique of the MBS564013 kit is based on C3 antibody-C3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
C3 elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the C3 present in samples reacts with the anti-C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C3 in the test sample. The quantity of C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: A number of serum proteins participate in acute inflammatory reactions. These include the complement, coagulation and kinin systems as well as a number of other proteins, known as acute phase proteins, that regulate acute inflammation.The complement system is a complex set of up to 20 serum proteins. The most abundant and pivotal of the complement components is C3, which has a molecular weight of about 187 kD and consists of an alpha and beta chain.
Product Categories/Family for C3 elisa kit
Sample Manual Insert of MBS564013. Click to request current manual
NCBI/Uniprot data below describe general gene information for C3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Official Full Name
C3
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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