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Global DNA Methylation ELISA Kit

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Catalog # MBS168205
Unit / Price
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  96 Assays  /  $605 +1 FREE 8GB USB
  5x96 Assays  /  $2,385 +2 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Global DNA Methylation, ELISA Kit

Popular Item
Full Product Name

Global DNA Methylation ELISA Kit (5'-methyl-2'-deoxycytidine Quantitation)

Product Synonym Names
Global DNA Methylation ELISA Kit (5'-methyl-2'-deoxycytidine Quantitation)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Assay Type
Quantitative Competitive
Preparation and Storage
Upon receipt, aliquot and store the 5MedCyd Standard and 5MedCyd DNA Conjugate at -20 degree C to avoid multiple freeze/thaw cycles. Store all other components at 4 degree C until their expiration dates.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Global DNA Methylation elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGlobal DNA Methylationpurchase
MBS168205 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Global DNA Methylation, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Global DNA Methylation. The ELISA analytical biochemical technique of the MBS168205 kit is based on Global DNA Methylation antibody-Global DNA Methylation antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Global DNA Methylation antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Global DNA Methylation. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Global DNA Methylation elisa kit
Principle of the Assay: The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5MedCyd. The unknown 5MedCyd samples or 5MedCyd standards are first added to the 5MedCyd DNA Conjugate Plate. After a brief incubation, an Anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.

Background: Epigenetic changes refer to stable, heritable, and reversible modifications. DNA methylation is one such epigenetic change and has been shown to be associated with almost every biological process. DNA methylation can increase the functional complexity of prokaryotic and eukaryotic genomes by providing additional avenues for the control of cellular processes. DNA methylation is also dynamic and thus can control the timing of cellular events. In prokaryotes, DNA methylation occurs at the C-5 or N-4 positions of cytosine, as well as the N-6 position of adenine. Multiple enzymes that are capable of methylating bacterial DNA have been identified and are named DNA-MTases. Initially, DNA methylation in bacteria was reported to be associated with restriction-modification systems, wherein foreign DNA that lacks DNA methylation can be recognized and degraded by host methylation-sensitive restriction enzymes. Further studies in bacteria also implicated DNA methylation in the maintenance of the DNA replication fidelity, gene expression regulation, and virulence. In plants, DNA methylation is found in the sequence context of CpG or CpNpG and has been implicated in normal plant development and regulation of transcription and transposition. At least two classes of DNA methyltransferases (DNMTs) have been identified and characterized in plants. These include the MET1 family of methyltransferases, which preferentially methylate cytosine in CpG context and function both as de novo and maintenance DNMTs, and a second class of DNMTs, named chromomethylases (CMTs), which are unique to plants and methylate cytosine in a CpNpG sequence context. In mammalian cells, DNA methylation is predominantly found at CpG dinucleotides, most of which reside within gene promoter regions, but in some instances, such as in the case of mouse and human embryonic stem cells, DNA methylation can also be found in non-CpG contexts. DNA methylation in mammalian cells is associated with repression of transcription and maintenance of genomic stability. Due to its important role in genome maintenance and other biological processes, deregulation of DNA methylation is associated with multiple human diseases including cancer. Three major enzymes are required for either de novo methylation (DNMT3A and DNMT3B) or maintenance methylation (DNMT1) in mammalian cells. There are several methods of detecting total 5-methylcytosine content in the genome. DNA can be digested into single nucleotides and total genomic 5-methylcytosine can be quantitated by high performance liquid chromatography, thin-layer chromatography, or liquid chromatography/mass spectroscopy, but all of these methods are labor intensive and technically demanding. Global DNA Methylation ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 5’-methyl-2’-deoxycytidine (5MedCyd) in urine directly, or extracted cell or tissue DNA samples. The quantity of 5MedCyd in an unknown sample is determined by comparing its absorbance with that of a known 5MedCyd standard curve. The kit has a 5MedCyd detection sensitivity range of 300 nM to 40,000 nM. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

Typical Testing Data/Standard Curve (for reference only) of Global DNA Methylation elisa kit
Global DNA Methylation elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS168205. Click to request current manual
Product References and Citations for Global DNA Methylation elisa kit
1. Robertson, K.D. (2005) Nat. Rev. Genet. 6:597-610.
2. Low, D.A., N.J. Weyand, and M.J. Mahan (2001) Infect. Immun. 69:7197-7204.
3. Jones, P.A., and S.B. Baylin (2007) Cell 128:683-692.
4. Noyer-Weidner, M., and T.A. Trautner (1993) EXS 64:39-108.
5. Finnegan, E.J., R.K. Genger, W.J. Peacock, and E.S. Dennis (1998) Annu. Rev. Plant Physiol. Plant Mol. Biol. 49:223-247.
6. Zilberman, D., M. Gehring, R.K. Tran, T. Ballinger, and S. Henikoff (2007) Nat. Genet. 39:61-69.
7. Finnegan, E.J., and K.A. Kovac (2000) Plant Mol. Biol. 43:189-201.
8. Ramsahoye, B.H., D. Biniszkiewicz, F. Lyko, V. Clark, A.P. Bird, and R. Jaenisch (2000) Proc. Natl. Acad. Sci. USA 97:5237-5242.
9. Lister, R., M. Pelizzola, R.H. Dowen, R.D. Hawkins, G. Hon, J. Tonti-Filippini, J.R. Nery, L. Lee (2009) Nature 462:315-322.
10. Chen, T., and E. Li (2004) Curr. Top. Dev. Biol. 60:55-89.
Jefferson, W. et al. (2013). Persistently Altered Epigenetic Marks in the Mouse Uterus After Neonatal Estrogen Exposure. Mol Endocrinol. 27:1666-1677.

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