• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Mouse IgG2A ELISA Kit

Scan QR to view Datasheet
Catalog # MBS564078
Unit / Price
Scan QR to view Datasheet
  1x96 Wells  /  $345 +1 FREE 8GB USB
Mouse IgG2A elisa kit
Product Name

Mouse IgG2A, ELISA Kit

Popular Item
Full Product Name

Mouse IgG2A ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Host
Mouse
Samples
Immunoperoxidase Assay for Determination of IgG2A in Mouse Samples
Assay Type
Sandwich
Intended Uses
The IgG2A test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG2A in biological samples of mice.
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Mouse IgG2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Mouse IgG2Apurchase
MBS564078 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mouse Mouse IgG2A, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Mouse IgG2A. The ELISA analytical biochemical technique of the MBS564078 kit is based on Mouse IgG2A antibody-Mouse IgG2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Mouse IgG2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Mouse IgG2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Mouse IgG2A elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgG2A present in samples reacts with the anti-IgG2A antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG2A antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG2A. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG2A in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG2A in the test sample. The quantity of IgG2A in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Product Categories/Family for Mouse IgG2A elisa kit
Sample Manual Insert of MBS564078. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000