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OxiSelect MDA Adduct ELISA Kit

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Catalog # MBS169156
Unit / Price
  32 Assays  /  $415 +1 FREE 8GB USB
  96 Assays  /  $770 +1 FREE 8GB USB
  5x96 Assays  /  $3,065 +3 FREE 8GB USB
Product Name

OxiSelect MDA Adduct, ELISA Kit

Popular Item
Full Product Name

OxiSelect MDA Adduct Competitive ELISA Kit

Product Synonym Names
OxiSelect MDA Adduct Competitive ELISA Kit
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Preparation and Storage
Upon receipt, aliquot and store the Anti-MDA Antibody, MDA-BSA Standard, MDA Conjugate and 100X Conjugate Diluent at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of OxiSelect MDA Adduct elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forOxiSelect MDA Adductpurchase
MBS169156 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OxiSelect MDA Adduct, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OxiSelect MDA Adduct. The ELISA analytical biochemical technique of the MBS169156 kit is based on OxiSelect MDA Adduct antibody-OxiSelect MDA Adduct antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OxiSelect MDA Adduct antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OxiSelect MDA Adduct. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
OxiSelect MDA Adduct elisa kit

Principle of the assay: First, an MDA conjugate is coated on an ELISA plate. The unknown MDA protein samples or MDA -BSA standards are then added to the MDA conjugate preabsorbed ELISA plate. After a brief incubation, an anti-MDA polyclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of MDA protein adducts in unknown samples is determined by comparison with a predetermined MDA-BSA standard curve.

Background: Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural bi-products of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative stress. Both MDA and HNE have been shown to be capable of binding to proteins and forming stable adducts, also termed advanced lipid peroxidation end products. These modifications of proteins by MDA or HNE can cause both structural and functional changes of oxidized proteins. The OxiSelect MDA Adduct Competitive ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MDA-protein adducts. The quantity of MDA adduct in protein samples is determined by comparing its absorbance with that of a known MDA-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

OxiSelect MDA Adduct elisa kit Testing Data #2 image
Sample Manual Insert of MBS169156. Click to request current manual
Product References and Citations for OxiSelect MDA Adduct elisa kit
1. Hoff HF, O'Neil J. (1993) J Lipid Res. 34: 1209-17.
2. Armstrong, D. and Browne, R. (1994). Free Radicals in Diagnostic Medicine. 366: 43-58.
3. Armstrong, D., et al. (1998). Free Radicals and Antioxidant Protocols. 108: 315-324.
4. Boyum, A. (1966). J. of Clinical Investigation. 21: Supplement 97.
5. Braun, D. and Fromherz, P. (1997). Applied Physics A.
6. Gidez, L., et al. (1982). J. of Lipid Research. 23: 1206-1223.
7. Lef'evre G., et al. (1998). Annals de Biologie Clinique. 56(3): 305-319.
8. Ohkawa, H., et al. (1979). Anal. Biochem. 95: 351-358.
9. Yagi, K. (1998). Free Radicals and Antioxidant Protocols. 108: 101-106.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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