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Scavenger Receptor Class B Member 1 (SRB1) ELISA Kit

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Catalog # MBS168982
Unit / Price
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  96 Assays  /  $600 +1 FREE 8GB USB
Product Name

Scavenger Receptor Class B Member 1 (SRB1), ELISA Kit

Full Product Name

Scavenger Receptor Class B Member 1 (SRB1) ELISA Kit

Product Synonym Names
Scavenger Receptor Class B Member 1 (SRB1) ELISA Kit
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Samples
Plasma, serum, cell or tissue lysate samples
Preparation and Storage
Upon receipt, aliquot and store the Human SRB1 Standard at -20 degree C to avoid multiple freeze/thaw cycles. Store all other components at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Scavenger Receptor Class B Member 1 (SRB1) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Scavenger Receptor Class B Member 1 (SRB1)purchase
MBS168982 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Scavenger Receptor Class B Member 1 (SRB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Scavenger Receptor Class B Member 1 (SRB1). The ELISA analytical biochemical technique of the MBS168982 kit is based on Scavenger Receptor Class B Member 1 (SRB1) antibody-Scavenger Receptor Class B Member 1 (SRB1) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Scavenger Receptor Class B Member 1 (SRB1) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Scavenger Receptor Class B Member 1 (SRB1). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Background: Cholesterol is an essential component of cellular membranes, as well as an essential substrate for steroid hormones and bile acid synthesis. However, cholesterol is toxic when accumulated in excess in cellular membranes, and elaborate pathways have evolved to control its synthesis, uptake and storage. Cells can obtain cholesterol either from de novo synthesis or uptake from circulating lipoproteins. While the low-density lipoprotein receptor (LDLR) is the primary pathway for removal of cholesterol from circulation, cholesterol within high density lipoprotein (HDL) is also removed from circulation through binding and endocytosis of the Scavenger Receptor Class B Member 1 (SRB1). SRB1 is a tetrameric transmembrane protein of 509 amino acids. SRB1 plays a critical role in the reverse cholesterol transport pathway (Figure 1), where cholesterol from macrophages and peripheral tissues is cleared and transported to the liver. While SRB1 has been shown to mediate binding of HDL and selective uptake of cholesteryl esters (CEs), the mechanism of CE uptake is not entirely clear. Unlike LDLR, which mediates endocytosis of the entire LDL particle into vesicles, the prevailing view for SRB1 is that it mediates HDL-CE transfer from the hydrophobic core of HDL without the internalization and subsequent degradation of the lipoprotein particle. In addition to its role in movement of CE, SRB1 overexpression has been shown to alter plasma membrane composition itself, increasing the levels of cholesterol within the plasma membrane. Scavenger Receptor Class B Member 1 (SRB1) ELISA Kit is an enzyme immunoassay for the detection and quantitation of human, mouse, rat, or hamster SRB1 in plasma, serum, cell or tissue lysate samples. The kit has a detection sensitivity limit of 600 pg/mL human SRB1. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

Sample Manual Insert of MBS168982. Click to request current manual
Protein Family
Product References and Citations for Scavenger Receptor Class B Member 1 (SRB1) elisa kit
1. Brown M. S., and Goldstein J. L. (1986). Science 232, 34-47.
2. Brown M. S., and Goldstein J. L. (1997). Cell 89, 331-340.
3. Han C-H. and Lee M-H. (2002). J. Vet. Sci. 3:265-272.
4. De la Llera-Moya M., Rothblat G.H., Connelly M.A., Kellner-Weibel G., Sakr S.W., Phillips M.C., and Willisma D.L. (1999) J. Lipid Res 40:575-580
5. Kent A.P. and Stylianou I.M. (2011) Hep. Med. Evid. Res. 3:29-44.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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