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ENA-78 elisa kit :: Rat ENA-78 ELISA Kit

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Catalog # MBS2512820
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ENA-78, ELISA Kit

Popular Item
Full Product Name

Rat ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600324
3D Structure
ModBase 3D Structure for P42830
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat ENA-78. No significant cross-reactivity or interference between Rat ENA-78 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat ENA-78 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ENA-78 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forENA-78purchase
MBS2512820 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ENA-78, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENA-78. The ELISA analytical biochemical technique of the MBS2512820 kit is based on ENA-78 antibody-ENA-78 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENA-78 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENA-78. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ENA-78 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ENA-78 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ENA-78. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ENA-78 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ENA-78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ENA-78. You can calculate the concentration of Rat ENA-78 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ENA-78 elisa kit
ENA-78 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2512820. Click to request current manual
NCBI/Uniprot data below describe general gene information for ENA-78. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,972 Da
NCBI Official Full Name
ENA-78
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 5
NCBI Official Symbol
CXCL5  [Similar Products]
NCBI Official Synonym Symbols
SCYB5; ENA-78
  [Similar Products]
NCBI Protein Information
C-X-C motif chemokine 5; epithelial-derived neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
UniProt Protein Name
C-X-C motif chemokine 5
UniProt Synonym Protein Names
ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5Cleaved into the following 2 chains:ENA-78(8-78); ENA-78(9-78)
UniProt Gene Name
CXCL5  [Similar Products]
UniProt Synonym Gene Names
ENA78; SCYB5  [Similar Products]
UniProt Entry Name
CXCL5_HUMAN
NCBI Summary for ENA-78
This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]
UniProt Comments for ENA-78
CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; chemotaxis; inflammatory response; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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