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ENO1 elisa kit :: Human enolase 1, (alpha) ELISA Kit

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Catalog # MBS706020
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

enolase 1, (alpha) (ENO1), ELISA Kit

Popular Item
Also Known As

Human Alpha-enolase, ENO1/ENO1L1/MBPB1/MPB1 ELISA Kit

Product Synonym Names
Human Alpha-enolase (ENO1/ENO1L1/MBPB1/MPB1) ELISA kit; ENO1L1; MBP-1; MPB1; NNE; PPH; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; enolase 1; non-neural enolase; phosphopyruvate hydratase; tau-crystallin; enolase 1; (alpha)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS706020 Testing Data
Sequence Length
341
OMIM
172430
3D Structure
ModBase 3D Structure for P06733
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human ENO1/ENO1L1/MBPB1/MPB1. No significant cross-reactivity or interference between human ENO1/ENO1L1/MBPB1/MPB1 and analogues was observed.
Samples
Serum, tissue homogenates, plasma.
Assay Type
Sandwich (Quantitative)
Detection Range
0.312 ng/ml-20 ng/ml.
Sensitivity
0.078 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ENO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forENO1purchase
MBS706020 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the enolase 1, (alpha) (ENO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENO1. The ELISA analytical biochemical technique of the MBS706020 kit is based on ENO1 antibody-ENO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ENO1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ENO1/ENO1L1/MBPB1/MPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ENO1/ENO1L1/MBPB1/MPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ENO1/ENO1L1/MBPB1/MPB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ENO1/ENO1L1/MBPB1/MPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ENO1 elisa kit
ENO1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS706020. Click to request current manual
NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,169 Da
NCBI Official Full Name
c-myc promoter-binding protein-1 isoform MBP-1
NCBI Official Synonym Full Names
enolase 1, (alpha)
NCBI Official Symbol
NCBI Official Synonym Symbols
NNE; PPH; MPB1; ENO1L1
  [Similar Products]
NCBI Protein Information
alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
UniProt Protein Name
Alpha-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ENO1L1; MBPB1; MPB1; NNE  [Similar Products]
UniProt Entry Name
ENOA_HUMAN
NCBI Summary for ENO1
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
UniProt Comments for ENO1
Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.5 PublicationsManual assertion based on experiment in:Ref.3

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.1 PublicationManual assertion based on experiment in:Ref.18

Cofactor: Magnesium. Binds two Mg2+ per subunit. Required for catalysis and for stabilizing the dimer.1 PublicationManual assertion based on experiment in:Ref.39
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withENO1 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with ENO1 and FLNA
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