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ENO3 elisa kit :: Human enolase 3 (beta, muscle) ELISA Kit

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Catalog # MBS931098
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ENO3 elisa kit
Product Name

enolase 3 (beta, muscle) (ENO3), ELISA Kit

Popular Item
Full Product Name

Human Beta-enolase, ENO3 ELISA Kit

Product Synonym Names
Human Beta-enolase (ENO3) ELISA kit; GSD13; MSE; 2-phospho-D-glycerate hydrolyase; enolase 3; muscle specific enolase; skeletal muscle enolase; enolase 3 (beta; muscle)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 612932
3D Structure
ModBase 3D Structure for P13929
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human ENO3. No significant cross-reactivity or interference between human ENO3 and analogues was observed.
Samples
serum, plasma, tissue homogenates.
Assay Type
Sandwich (Quantitative)
Detection Range
0.625 ng/ml-40 ng/ml.
Sensitivity
Typically less than 0.156 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ENO3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ENO3purchase
MBS931098 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the enolase 3 (beta, muscle) (ENO3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENO3. The ELISA analytical biochemical technique of the MBS931098 kit is based on ENO3 antibody-ENO3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENO3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENO3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Sample Manual Insert of MBS931098. Click to request current manual
NCBI/Uniprot data below describe general gene information for ENO3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,987 Da
NCBI Official Full Name
beta-enolase isoform 2
NCBI Official Synonym Full Names
enolase 3 (beta, muscle)
NCBI Official Symbol
NCBI Official Synonym Symbols
MSE; GSD13
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NCBI Protein Information
beta-enolase; muscle-specific enolase; skeletal muscle enolase; 2-phospho-D-glycerate hydrolyase
UniProt Protein Name
Beta-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ENOB_HUMAN
NCBI Summary for ENO3
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]
UniProt Comments for ENO3
ENO3: Appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen- beta particles are detected on ultrastructural analysis. Belongs to the enolase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.2.1.11; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: extracellular space; phosphopyruvate hydratase complex; cytoplasm; plasma membrane; cytosol

Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: response to drug; glycolysis; carbohydrate metabolic process; glucose metabolic process; skeletal muscle regeneration; pathogenesis; aging; gluconeogenesis

Disease: Glycogen Storage Disease Xiii
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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