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anti-eNOS antibody :: eNOS Antibody

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Catalog # MBS852269
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Western Blot (WB)
Product Name

eNOS, Antibody

Popular Item
Also Known As

eNOS (Phospho-Ser615) Antibody

Product Synonym Names
Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; NOS; type III; NOS3; NOSIII; Nitric-oxide synthase; endothelial; cNOS
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1203
OMIM
163729
3D Structure
ModBase 3D Structure for P29474
Species Reactivity
Human, Mouse, Rat
Specificity
eNOS (Phospho-Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 615 (F-N-SP-I-S).
All Sites
Human: Ser615
Mouse: Ser614
Rat: Ser614
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-eNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-eNOS antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-eNOS antibody
WB: 1:500~1:1000
ELISA: 1:40000

Western Blot (WB) of anti-eNOS antibody
Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.
anti-eNOS antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,862 Da
NCBI Official Full Name
nitric oxide synthase, endothelial isoform 1
NCBI Official Synonym Full Names
nitric oxide synthase 3
NCBI Official Symbol
NCBI Official Synonym Symbols
eNOS; ECNOS
  [Similar Products]
NCBI Protein Information
nitric oxide synthase, endothelial
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Synonym Protein Names
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
UniProt Gene Name
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII  [Similar Products]
NCBI Summary for eNOS
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
UniProt Comments for eNOS
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Product References and Citations for anti-eNOS antibody
Janssens S.P., J. Biol. Chem. 267:14519-14522(1992). Janssens S.P., J. Biol. Chem. 267:22694-22694(1992). Marsden P.A., FEBS Lett. 307:287-293(1992).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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