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entA recombinant protein :: 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (entA) Recombinant Protein

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Catalog # MBS1118942
Unit / Price
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  0.05 mg (E-Coli)  /  $650 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $770 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $875 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $960 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $1,000 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $1,040 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,180 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,240 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,445 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,445 +1 FREE 8GB USB
  1 mg (Yeast)  /  $1,870 +2 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $1,890 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $2,020 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $2,930 +3 FREE 8GB USB
entA recombinant protein
Product Name

2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (entA), Recombinant Protein

Full Product Name

Recombinant Escherichia coli 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (entA)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-248aa, full length protein
Sequence
MDFSGKNVWV TGAGKGIGYA TALAFVEAGA KVTGFDQAFT QEQYPFATEV MDVADAAQVA QVCQRLLAET ERLDALVNAA GILRMGATDQ LSKEDWQQTF AVNVGGAFNL FQQTMNQFRR QRGGAIVTVA SDAAHTPRIG MSAYGASKAA LKSLALSVGL ELAGSGVRCN VVSPGSTDTD MQRTLWVSDD AEEQRIRGFG EQFKLGIPLG KIARPQEIAN TILFLASDLA SHITLQDIVV DGGSTLGA
3D Structure
ModBase 3D Structure for P15047
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Escherichia coli (strain K12)
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of entA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for entA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
26,250 Da
NCBI Official Full Name
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
ECK0589; JW0588
  [Similar Products]
NCBI Protein Information
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
UniProt Protein Name
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
UniProt Synonym Protein Names
Trans-2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
DiDHB-DH  [Similar Products]
NCBI Summary for entA
2,3-Dihydro-2,3-dihydroxybenzoate dehydrogenase catalyzes the formation of 2,3-dihydroxybenzoate (DHB), an intermediate in the enterobactin biosynthesis pathway. [More information is available at EcoCyc: EG10259].
UniProt Comments for entA
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. Catalyzes the reversible NAD-dependent oxidation of the C3-hydroxyl group of 2,3-dihydro-2,3-dihydroxybenzoate (2,3-diDHB), producing the transient intermediate 2-hydroxy-3-oxo-4,6-cyclohexadiene-1-carboxylate, which undergoes rapid aromatization to the final product, 2,3-dihydroxybenzoate (2,3-DHB). Only the compounds with a C3-hydroxyl group such as methyl 2,3-dihydro-2,3-dihydroxybenzoate, methyl-3-hydroxy-1,4-cyclohexadiene-1-carboxylate, trans-3-hydroxy-2-cyclohexene-1-carboxylate, cis-3-hydroxy-4-cyclohexene-1-carboxylate, cis-3-hydroxycyclohexane-1-carboxylic acid are oxidized to the corresponding ketone products. The stereospecificity of the C3 allylic alcohol group oxidation is 3R in a 1R,3R dihydro substrate. It can also increase the DHB-AMP ligase activity of EntE by interaction EntE.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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