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entB recombinant protein :: Isochorismatase (entB) Recombinant Protein

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Catalog # MBS1019667
Unit / Price
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  0.05 mg (E-Coli)  /  $690 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $800 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $925 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,015 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $1,035 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $1,085 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,230 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,275 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,490 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,535 +1 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $1,945 +2 FREE 8GB USB
  1 mg (Yeast)  /  $1,950 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $2,070 +2 FREE 8GB USB
  1 mg (Baculovirus)  /  $3,015 +3 FREE 8GB USB
entB recombinant protein
Product Name

Isochorismatase (entB), Recombinant Protein

Full Product Name

Recombinant Escherichia coli Isochorismatase (entB)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-285aa, full length protein
Sequence
MAIPKLQAYA LPESHDIPQN KVDWAFEPQR AALLIHDMQD YFVSFWGENC PMMEQVIANI AALRDYCKQH NIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQKVVD RLTPDADDTV LVKWRYSAFH RSPLEQMLKE SGRNQLIITG VYAHIGCMTT ATDAFMRDIK PFMVADALAD FSRDEHLMSL KYVAGRSGRV VMTEELLPAP IPASKAALRE VILPLLDESD EPFDDDNLID YGLDSVRMMA LAARWRKVHG DIDFVMLAKN PTIDAWWKLL SREVK
3D Structure
ModBase 3D Structure for P0ADI4
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Escherichia coli (strain K12)
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of entB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for entB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
32,554 Da
NCBI Official Full Name
isochorismatase
NCBI Official Symbol
NCBI Official Synonym Symbols
ECK0588; entG; JW0587
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NCBI Protein Information
isochorismatase
UniProt Protein Name
Enterobactin synthase component B
UniProt Synonym Protein Names
2,3-dihydro-2,3-dihydroxybenzoate synthase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for entB
Recent gel filtration data suggest that EntB may be a trimer not a pentamer. [More information is available at EcoCyc: EG10260].
UniProt Comments for entB
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron2+ atoms. EntB is a bifunctional protein that serves as an isochorismate lyase and an aryl carrier protein (ArCP). Catalyzes the conversion of isochorismate to 2,3-dihydro-2,3-dihydroxybenzoate (2,3-diDHB), the precursor of 2,3-dihydroxybenzoate (DHB). In the enterobactin assembly, EntB functions as an aryl carrier protein phosphopantetheinylated near the C terminus by EntD to yield holo-EntB, which is then acylated by EntE with 2,3-dihydroxybenzoyl-AMP to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2,3-dihydroxybenzoate (DHB) and L-serine.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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