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ENTPD1 recombinant protein :: Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) Recombinant Protein

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Catalog # MBS2018714 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $265 +1 FREE 8GB USB
  0.1 mg  /  $405 +1 FREE 8GB USB
  0.2 mg  /  $500 +1 FREE 8GB USB
  0.5 mg  /  $970 +1 FREE 8GB USB
  1 mg  /  $1,435 +1 FREE 8GB USB
SDS-Page
Product Name

Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1), Recombinant Protein

Also Known As

Recombinant Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
510
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TDE IGAYLAECME LSTELIPTSK HHQTPVYLGA TAGMRLLRME SEQSADEVLA AVSTSLKSYP FDFQGAKIIT GQEEGAYGWI TINYLLGRFT QEQSWLSLIS DSQKQETFGA LDLGGASTQI TFVPQNSTIE SPENSLQFRL YGEDYTVYTH SFLCYGKDQA LWQKLAKDIQ VSSGGVLKDP CFNPGYEKVV NVSELYGTPC TKRFEKKLPF DQFRIQGTGD YEQCHQSILE LFNNSHCPYS QCAFNGVFLP PL
Chromosome Location
Chromosome: 19; NC_000085.6 (40612363..40741602). Location: 19 C3; 19 34.25 cM
3D Structure
ModBase 3D Structure for P55772
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Thr98~Leu352 (Accession # P55772) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Membrane; Multi-pass membrane protein
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
5.5
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of ENTPD1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ENTPD1 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for ENTPD1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

NCBI/Uniprot data below describe general gene information for ENTPD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
32.3kDa
NCBI Official Full Name
ectonucleoside triphosphate diphosphohydrolase 1
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 1
NCBI Official Symbol
Entpd1  [Similar Products]
NCBI Official Synonym Symbols
Cd39; AA408691; NTPDase-1; 2610206B08Rik
  [Similar Products]
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; ectoapyrase; CD39 antigen; ecto-apyrase; ecto-ATPase 1; ecto-ATPDase 1; ecto-ATP diphosphohydrolase 1; lymphoid cell activation antigen
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 1
UniProt Synonym Protein Names
Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen
UniProt Gene Name
Entpd1  [Similar Products]
UniProt Synonym Gene Names
Cd39; NTPDase 1; Ecto-ATPDase 1  [Similar Products]
UniProt Entry Name
ENTP1_MOUSE
UniProt Comments for ENTPD1
ENTPD1: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Membrane protein, integral; Nucleotide Metabolism - purine; EC 3.6.1.5; Membrane protein, multi-pass; Hydrolase; Nucleotide Metabolism - pyrimidine

Cellular Component: extracellular space; synaptic vesicle; neuron projection; cell surface; membrane; cell soma; basolateral plasma membrane; postsynaptic density; integral to membrane; basal lamina

Molecular Function: identical protein binding; hydrolase activity; nucleoside-diphosphatase activity; ATPase activity; nucleotide binding; nucleoside-triphosphatase activity; ATP binding; adenosine-diphosphatase activity

Biological Process: G-protein coupled receptor protein signaling pathway; platelet activation; metabolic process; purine ribonucleoside diphosphate catabolic process; negative regulation of dopamine secretion; protein homooligomerization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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