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Deoxyribonuclease I Enzyme

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Catalog # MBS653701
Unit / Price
  5 mg  /  $175 +1 FREE 8GB USB
  25 mg  /  $355 +1 FREE 8GB USB
  100 mg  /  $960 +1 FREE 8GB USB
Deoxyribonuclease I enzyme
Product Name

Deoxyribonuclease I, Enzyme

Popular Item
Full Product Name

Deoxyribonuclease I (DNase I), >=1500u/mg, Lyophilized; EC=3.1.21.1; 5'-Oligonucleotidohydrolase

Product Synonym Names
Deoxyribonuclease I (DNase I), 1500u/mg, Lyophilized
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a chromatographically purified, salt-free, off-white lyophilized powder.
Source
Bovine pancreas
CAS Number
9003-98-9
Activity
> or = 1500u/mg

Lot Specific:
2661u/mg
2862u/E280
Extinction Coefficient
E1%, 280nm = 9.3
Reconstitution
Reconstitute in 0.15M sodium chloride solution at 2mg/ml. Yields a clear, colorless solution.
Country of Origin
The raw animal product used in the manufacturing of Deoxyribonuclease I (DNase I) is derived from animal material originating in New Zealand, Uruguian or Brazil where there have been no reported cases of bovine spongiform encephalopathy (BSE). During processing, the product is subjected to a pH of 1.8 for a minimum period of 14 hours.
Unit Definition
1 unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0.
Note: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37 degree C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of Deoxyribonuclease I enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Deoxyribonuclease I enzyme
Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3'-hydroxyl oligonucleotides. Supplied as a chromatographically purified, lyophilized powder.
Application Notes for Deoxyribonuclease I enzyme
Nick Translation, DNase I DNA Footprinting, Cloning of Random DNA Fragments
NCBI/Uniprot data below describe general gene information for Deoxyribonuclease I. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
deoxyribonuclease I
UniProt Comments for Deoxyribonuclease I
DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.

Protein type: Secreted, signal peptide; EC 3.1.21.1; Secreted; Deoxyribonuclease; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region; nuclear envelope; nucleus

Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity

Biological Process: apoptosis; DNA catabolic process, endonucleolytic

Disease: Systemic Lupus Erythematosus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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