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EPAS1 elisa kit :: Human endothelial PAS domain protein 1 ELISA Kit

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Catalog # MBS702348
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

endothelial PAS domain protein 1 (EPAS1), ELISA Kit

Popular Item
Also Known As

Human hypoxia-inducible factor 2, HIF-2 ELISA Kit

Product Synonym Names
Human hypoxia-inducible factor 2 (HIF-2) ELISA kit; ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73; HIF-1alpha-like factor; basic-helix-loop-helix-PAS protein MOP2; hypoxia-inducible factor 2 alpha; member of PAS protein 2; endothelial PAS domain protein 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS702348 Testing Data
Sequence Length
870
OMIM
gene 611783
3D Structure
ModBase 3D Structure for Q99814
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human HIF-2. No significant cross-reactivity or interference between human HIF-2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/ml -4000 pg/ml
Sensitivity
Typically less than 15.6 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EPAS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEPAS1purchase
MBS702348 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the endothelial PAS domain protein 1 (EPAS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EPAS1. The ELISA analytical biochemical technique of the MBS702348 kit is based on EPAS1 antibody-EPAS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPAS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPAS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EPAS1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of EPAS1 elisa kit
EPAS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS702348. Click to request current manual
NCBI/Uniprot data below describe general gene information for EPAS1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
96,459 Da
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
EPAS1  [Similar Products]
NCBI Official Synonym Symbols
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
  [Similar Products]
NCBI Protein Information
endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Synonym Protein Names
Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
UniProt Gene Name
EPAS1  [Similar Products]
UniProt Synonym Gene Names
BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha  [Similar Products]
UniProt Entry Name
EPAS1_HUMAN
NCBI Summary for EPAS1
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
UniProt Comments for EPAS1
Function: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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