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anti-EPHA2/3 antibody :: anti-Human, Mouse EPHA2/3 Antibody

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Catalog # MBS854845
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

EPHA2/3, Antibody

Popular Item
Also Known As

EPHA2/3 (Phospho-Tyr588/596) Antibody

Product Synonym Names
EC 2.7.10.1; ECK; Ephrin type-A receptor 2 precursor; Epithelial cell kinase; MPK-5; SEK-2; SEK2; Tyrosine-protein kinase receptor ECK; kinase EphA2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
976
OMIM
116600
3D Structure
ModBase 3D Structure for P29317
Species Reactivity
Human, Mouse
Specificity
EPHA2/3/4 (Phospho-Tyr588/596) antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human EPHA2/3/4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).
All Sites
Human: Tyr588/Tyr596/Tyr596
Mouse: Tyr589/Tyr596/Tyr596
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-EPHA2/3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EPHA2/3 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-EPHA2/3 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000

Testing Data of anti-EPHA2/3 antibody
anti-EPHA2/3 antibody Testing Data image
Western Blot (WB) of anti-EPHA2/3 antibody
P-peptide-+ Western blot analysis of extracts from HepG2 cells, using EPHA2/3/4 (Phospho-Tyr588/596) antibody. Immunofluorescence analysis of HeLa cells, using EPHA2/3/4 (Phospho-Tyr588/596) antibody.
anti-EPHA2/3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for EPHA2/3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,305 Da
NCBI Official Full Name
ephrin type-A receptor 2 isoform 1
NCBI Official Synonym Full Names
EPH receptor A2
NCBI Official Symbol
EPHA2  [Similar Products]
NCBI Official Synonym Symbols
ECK; CTPA; ARCC2; CTPP1; CTRCT6
  [Similar Products]
NCBI Protein Information
ephrin type-A receptor 2
UniProt Protein Name
Ephrin type-A receptor 2
UniProt Synonym Protein Names
Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
UniProt Gene Name
EPHA2  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for EPHA2/3
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]
UniProt Comments for EPHA2/3
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
Product References and Citations for anti-EPHA2/3 antibody
Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990). Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005). Zhuang G., J. Biol. Chem. 282:2683-2694(2007).

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Disclaimer
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