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anti-EPHA4 antibody :: anti-Human, Mouse EPHA4 Antibody

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Catalog # MBS854677
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Western Blot (WB)
Product Name

EPHA4, Antibody

Popular Item
Full Product Name

EPHA4 (Phospho-Tyr596) Antibody

Product Synonym Names
Ephrin type-A receptor 4; EC=2.7.10.1; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602188
3D Structure
ModBase 3D Structure for P54764
Species Reactivity
Human, Mouse
Specificity
EPHA4 (Phospho-Tyr596) antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human EPHA4 around the phosphorylation site of tyrosine 596.
All Sites
Human: Tyr596
Mouse: Tyr596
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-EPHA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EPHA4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-EPHA4 antibody
WB: 1:500~1:1000
ELISA: 1:20000

Western Blot (WB) of anti-EPHA4 antibody
Western blot analysis of extracts from JurKat cells, using EPHA4 (Phospho-Tyr596) antibody.
anti-EPHA4 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for EPHA4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
104,507 Da
NCBI Official Full Name
ephrin type-A receptor 4 isoform a
NCBI Official Synonym Full Names
EPH receptor A4
NCBI Official Symbol
EPHA4  [Similar Products]
NCBI Official Synonym Symbols
SEK; HEK8; TYRO1
  [Similar Products]
NCBI Protein Information
ephrin type-A receptor 4
UniProt Protein Name
Ephrin type-A receptor 4
UniProt Synonym Protein Names
EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK
Protein Family
UniProt Gene Name
EPHA4  [Similar Products]
UniProt Synonym Gene Names
HEK8; SEK; TYRO1; EK8; hEK8  [Similar Products]
NCBI Summary for EPHA4
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
UniProt Comments for EPHA4
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
Product References and Citations for anti-EPHA4 antibody
Rikova K., Cell 131:1190-1203(2007) [PubMed: 18083107]. Richter M., J. Neurosci. 27:14205-14215(2007) [PubMed: 18094260]. Fu W.Y., Nat. Neurosci. 10:67-76(2007) [PubMed: 17143272].

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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