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EPX elisa kit :: Mouse eosinophil peroxidase ELISA Kit

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Catalog # MBS705531
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

eosinophil peroxidase (EPX), ELISA Kit

Popular Item
Also Known As

Mouse Eosinophil Peroxidase, EPO ELISA Kit

Product Synonym Names
Mouse Eosinophil Peroxidase (EPO) ELISA Kit; EPO; EPP; EPX-PEN; ; eosinophil peroxidase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS705531 Testing Data
Sequence Length
716
3D Structure
ModBase 3D Structure for P49290
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse EPO. No significant cross-reactivity or interference between mouse EPO and analogues was observed.
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich (Quantitative)
Detection Range
0.156 ng/ml-10 ng/ml.
Sensitivity
0.039 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EPX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEPXpurchase
MBS705531 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the eosinophil peroxidase (EPX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EPX. The ELISA analytical biochemical technique of the MBS705531 kit is based on EPX antibody-EPX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EPX elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of EPX elisa kit
EPX elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705531. Click to request current manual
NCBI/Uniprot data below describe general gene information for EPX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,380 Da
NCBI Official Full Name
eosinophil peroxidase
NCBI Official Synonym Full Names
eosinophil peroxidase
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
eosinophil peroxidase
UniProt Protein Name
Eosinophil peroxidase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Eper; EPO  [Similar Products]
UniProt Entry Name
PERE_MOUSE
UniProt Comments for EPX
Function: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils 1 PublicationManual assertion based on experiment in:Ref.4

Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor: Binds 1 calcium ion per heterodimer
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with EPX elisa kit
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