• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-ER-alpha antibody :: Mouse anti-Human ER-alpha Antibody

Scan QR to view Datasheet
Catalog # MBS850870
Unit / Price
  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

ER-alpha, Antibody

Full Product Name

ER-alpha Antibody

Product Synonym Names
ER; ESR1; Era; ESRA
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
133430
3D Structure
ModBase 3D Structure for P03372
Isotype
IgG1
Clone Number
2B7
Host
Mouse
Species Reactivity
Human
Form/Format
Purified antibody in PBS containing 0.03% sodium azide.
Immunogen
Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Other Notes
Small volumes of anti-ER-alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ER-alpha antibody
The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.
Applications Tested/Suitable for anti-ER-alpha antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-ER-alpha antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-ER-alpha antibody
Figure 1. Western blot analysis using ER-alpha mouse mAb against human breast carcinoma tissue lysate.
anti-ER-alpha antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ER-alpha antibody
Figure 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-alpha mouse mAb showing nuclear expression with DAB staining.
anti-ER-alpha antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ER-alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,547 Da
NCBI Official Full Name
estrogen receptor isoform 1
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; ESTRR; NR3A1
  [Similar Products]
NCBI Protein Information
estrogen receptor
UniProt Protein Name
Estrogen receptor
UniProt Synonym Protein Names
ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
UniProt Gene Name
UniProt Synonym Gene Names
ESR; NR3A1; ER  [Similar Products]
NCBI Summary for ER-alpha
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
UniProt Comments for ER-alpha
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000