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ERa elisa kit :: Human Estrogen Receptor Alpha (ERa) ELISA Kit

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Catalog # MBS2019576 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Estrogen Receptor Alpha (ERa), ELISA Kit

Popular Item
Full Product Name

Estrogen Receptor Alpha (ERa) ELISA Kit

Product Synonym Names
ER-A1; ER; ESR; ESR1; ESRA; NR3A1; NR3-A1; Estrogen Receptor 1; Nuclear Receptor Subfamily 3,Group A,Member 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Alpha (ERa).
No significant cross-reactivity or interference between Estrogen Receptor Alpha (ERa) and analogues was observed.
Assay Type
Double-antibody Sandwich
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
< 0.239ng/mL
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ERa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ERapurchase
MBS2019576 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estrogen Receptor Alpha (ERa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ERa. The ELISA analytical biochemical technique of the MBS2019576 kit is based on ERa antibody-ERa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ERa elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Alpha (ERa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Alpha (ERa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Alpha (ERa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Alpha (ERa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ERa elisa kit

Typical Testing Data/Standard Curve (for reference only) of ERa elisa kit
ERa elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2019576. Click to request current manual
NCBI/Uniprot data below describe general gene information for ERa. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
Molecular Weight
35,547 Da
NCBI Official Full Name
estrogen receptor alpha, partial
UniProt Protein Name
Estrogen receptor
UniProt Synonym Protein Names
ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ESR; NR3A1; ER  [Similar Products]
UniProt Entry Name
UniProt Comments for ERa
ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; steroid hormone mediated signaling; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gene expression; positive regulation of transcription from RNA polymerase II promoter

Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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