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anti-ERN1 antibody :: Mouse anti-Human ERN1 Antibody

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Catalog # MBS850823
Unit / Price
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  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

ERN1, Antibody

Full Product Name

ERN1 Antibody

Product Synonym Names
IRE1; IRE1P; IRE1a; hIRE1p; FLJ30999; MGC163277; MGC163279; ERN1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
604033
3D Structure
ModBase 3D Structure for O75460
Isotype
IgG1
Clone Number
9F2
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Immunogen
Purified recombinant fragment of human ERN1(aa282-433) expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
Other Notes
Small volumes of anti-ERN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ERN1 antibody
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.
Applications Tested/Suitable for anti-ERN1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-ERN1 antibody
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-ERN1 antibody
Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat (3), Hela(4) and HEK293 (5) cell lysate.
anti-ERN1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ERN1 antibody
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 mouse mAb with DAB staining.
anti-ERN1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ERN1 antibody
Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ERN1 mouse mAb
anti-ERN1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ERN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
109kDa
NCBI Official Full Name
serine/threonine-protein kinase/endoribonuclease IRE1
NCBI Official Synonym Full Names
endoplasmic reticulum to nucleus signaling 1
NCBI Official Symbol
NCBI Official Synonym Symbols
IRE1; IRE1P; IRE1a; hIRE1p
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Protein Name
Serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Synonym Protein Names
Endoplasmic reticulum-to-nucleus signaling 1
UniProt Gene Name
UniProt Synonym Gene Names
IRE1a  [Similar Products]
NCBI Summary for ERN1
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
UniProt Comments for ERN1
Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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