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ESM1 elisa kit :: Human Endocan ELISA Kit

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Catalog # MBS824849
Unit / Price
  96 Tests  /  $470 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Endocan (ESM1), ELISA Kit

Popular Item
Full Product Name

Human Endocan ELISA Kit

Product Synonym Names
Endothelial cell-specific molecule 1; ESM-1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601521
3D Structure
ModBase 3D Structure for Q9NQ30
Species Reactivity
Specificity
The Human Endocan ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Endocan proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
<10 pg/mL
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ESM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forESM1purchase
MBS824849 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endocan (ESM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ESM1. The ELISA analytical biochemical technique of the MBS824849 kit is based on ESM1 antibody-ESM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ESM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ESM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ESM1 elisa kit
Principle of the Assay: MBS ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endocan in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Endocan coated on a 96-well plate. Standards and samples are pipetted into the wells and Endocan present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endocan antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endocan bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
Applications Tested/Suitable for ESM1 elisa kit
Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only) of ESM1 elisa kit
ESM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS824849. Click to request current manual
NCBI/Uniprot data below describe general gene information for ESM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,513 Da
NCBI Official Full Name
endothelial cell-specific molecule 1 isoform b
NCBI Official Synonym Full Names
endothelial cell-specific molecule 1
NCBI Official Symbol
NCBI Official Synonym Symbols
endocan
  [Similar Products]
NCBI Protein Information
endothelial cell-specific molecule 1; ESM-1
UniProt Protein Name
Endothelial cell-specific molecule 1
UniProt Gene Name
UniProt Synonym Gene Names
ESM-1  [Similar Products]
UniProt Entry Name
ESM1_HUMAN
NCBI Summary for ESM1
This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Comments for ESM1
ESM1: May have potent implications in lung endothelial cell- leukocyte interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: integrin binding; insulin-like growth factor binding; hepatocyte growth factor receptor binding

Biological Process: positive regulation of cell proliferation; angiogenesis; regulation of cell growth; sprouting angiogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with ESM1 elisa kit
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