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EWSR1 elisa kit :: Human Ewing sarcoma breakpoint region 1 ELISA Kit

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Catalog # MBS908129
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Ewing sarcoma breakpoint region 1 (EWSR1), ELISA Kit

Popular Item
Also Known As

Human RNA-binding protein EWS, EWSR1 ELISA Kit

Product Synonym Names
Human RNA-binding protein EWS (EWSR1) ELISA kit; AC002059.7; EWS; bK984G1.4; Ewings sarcoma EWS-Fli1 (type 1) oncogene; RNA-binding protein EWS; Ewing sarcoma breakpoint region 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS908129 Testing Data
Sequence Length
655
OMIM
612219
3D Structure
ModBase 3D Structure for Q01844
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human EWSR1. No significant cross-reactivity or interference between human EWSR1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/ml -1600 pg/mL
Sensitivity
<6.25 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of EWSR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forEWSR1purchase
MBS908129 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ewing sarcoma breakpoint region 1 (EWSR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EWSR1. The ELISA analytical biochemical technique of the MBS908129 kit is based on EWSR1 antibody-EWSR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EWSR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EWSR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
EWSR1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EWSR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EWSR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EWSR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EWSR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of EWSR1 elisa kit
EWSR1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS908129. Click to request current manual
NCBI/Uniprot data below describe general gene information for EWSR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
68,478 Da
NCBI Official Full Name
RNA-binding protein EWS isoform 3
NCBI Official Synonym Full Names
EWS RNA-binding protein 1
NCBI Official Symbol
EWSR1  [Similar Products]
NCBI Official Synonym Symbols
EWS; bK984G1.4
  [Similar Products]
NCBI Protein Information
RNA-binding protein EWS; EWS RNA-binding protein variant 6; Ewing sarcoma breakpoint region 1; Ewings sarcoma EWS-Fli1 (type 1) oncogene
UniProt Protein Name
RNA-binding protein EWS
UniProt Synonym Protein Names
EWS oncogene; Ewing sarcoma breakpoint region 1 protein
Protein Family
UniProt Gene Name
EWSR1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
EWS_HUMAN
NCBI Summary for EWSR1
This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]
UniProt Comments for EWSR1
EWS: an oncogenic RNA-binding protein. Might normally function as a repressor. Binds RPB3, SF1, calmodulin and RNA. Interacts with and regulated by Pyk2. Relocates from cytoplasm to ribosomes upon Pyk2 activation. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. 5 alternatively-spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor; Oncoprotein; RNA-binding

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; plasma membrane; nucleus

Molecular Function: calmodulin binding; identical protein binding; protein binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

Disease: Ewing Sarcoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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