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F1+2 elisa kit :: Rat Prothrombin Fragment 1+2 (F1+2) ELISA Kit

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Catalog # MBS260130
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Prothrombin Fragment 1+2 (F1+2), ELISA Kit

Popular Item
Full Product Name

Rat Prothrombin Fragment 1+2 (F1+2) ELISA kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
614390
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant F1+2 concentration
Assay Type
Sandwich
Detection Range
2000 pg/ml-31.2 pg/ml
Sensitivity
12 pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of F1+2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forF1+2purchase
MBS260130 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prothrombin Fragment 1+2 (F1+2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F1+2. The ELISA analytical biochemical technique of the MBS260130 kit is based on F1+2 antibody-F1+2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F1+2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F1+2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Economical detection of cell sample based prothrombin fragment 1+2 may be accomplished with an ELISA kit product. Inactive prothrombin is enzymatically cleaved to active thrombin serine protease, a key coagulation factor XII in blood clotting cascades. Thus, concentration of human F1 2 is critical to prevent serious blood loss. Rat models provide a robust research avenue into clotting deficiencies involving fragment 1 + 2 and quantitation of rat samples by these ELISA products provide excellent assay precision and high intra assay reproducibility. These kits include all required reagents; primary antibody bound to ELISA kit 96t wells plate, wash solution reagent, secondary antibodies protein for standard curve in 1 vial, detection system for middle high level concentrations and stop solution for colorimetric read on plate reader. Besides rat specific ELISA kits, human F12 ELISA kits and guinea pig F1 2 ELISA to prothrombin fragment 1 with hu datasheets may be ordered.
Related Product Information for
F1+2 elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat F1+2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of F1+2 elisa kit
F1+2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS260130. Click to request current manual
NCBI/Uniprot data below describe general gene information for F1+2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70,037 Da
NCBI Official Full Name
prothrombin preproprotein
NCBI Official Synonym Full Names
coagulation factor II (thrombin)
NCBI Official Symbol
NCBI Official Synonym Symbols
PT; THPH1; RPRGL2
  [Similar Products]
NCBI Protein Information
prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II
UniProt Protein Name
Prothrombin
UniProt Synonym Protein Names
Coagulation factor IICleaved into the following 4 chains:Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain
UniProt Gene Name
UniProt Entry Name
THRB_HUMAN
NCBI Summary for F1+2
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq, Jul 2008]
UniProt Comments for F1+2
prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.5; Secreted; Protease; Secreted, signal peptide; Apoptosis

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: protein binding; growth factor activity; serine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: positive regulation of blood coagulation; multicellular organismal development; positive regulation of collagen biosynthetic process; proteolysis; regulation of cell shape; negative regulation of fibrinolysis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; response to wounding; acute-phase response; negative regulation of proteolysis; platelet activation; cytosolic calcium ion homeostasis; post-translational protein modification; positive regulation of cell growth; peptidyl-glutamic acid carboxylation; positive regulation of phosphoinositide 3-kinase cascade; fibrinolysis; cellular protein metabolic process; regulation of gene expression; negative regulation of astrocyte differentiation; regulation of blood coagulation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of protein amino acid phosphorylation; blood coagulation; blood coagulation, intrinsic pathway; leukocyte migration

Disease: Thrombophilia Due To Thrombin Defect; Stroke, Ischemic; Pregnancy Loss, Recurrent, Susceptibility To, 2; Prothrombin Deficiency, Congenital
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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