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F2 elisa kit :: Prothrombin total antigen assay ELISA Kit

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Catalog # MBS135499
Unit / Price
  1 Kit  /  $375 +1 FREE 8GB USB
  5 Kits  /  $1,490 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Prothrombin total antigen assay (F2), ELISA Kit

Popular Item
Also Known As

Human Prothrombin total antigen assay ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
622
Chromosome Location
Chromosome: 11; NC_000011.9 (46740743..46761056). Location: 11p11
OMIM
176930
3D Structure
ModBase 3D Structure for P00734
Host
Multiple
Specificity
This assay recognizes natural human total prothrombin (prothrombin, thrombin, and thrombin-antithrombin complex). Pooled normal plasma from mouse, pig, and canine were assayed for cross-reactivity. No significant cross-reactivity was observed.
Form/Format
Complete Kit
Samples
Human plasma
Sensitivity
The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.147-0.169) and calculating the corresponding concentration. The MDD was 0.166 ng/ml.
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of F2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forF2purchase
MBS135499 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple Prothrombin total antigen assay (F2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F2. The ELISA analytical biochemical technique of the MBS135499 kit is based on F2 antibody-F2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
F2 elisa kit
Intended Uses: This human prothrombin antigen assay is intended for the quantitative determination of total prothrombin antigen in human plasma. For research use only.

Principle of the Assay: Human prothrombin will bind to the capture antibody coated on the microtiter plate. Prothrombin, thrombin, and thrombin-antithrombin complex will react with the antibody on the plate. After appropriate washing steps, anti-human prothrombin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prothrombin. Color development is proportional to the concentration of total prothrombin in the samples.

Background: Prothrombin (aka Factor II) is a single-chain vitamin K-dependent 579 amino acid glycoprotein zymogen [1]. Prothrombin is proteolytically activated to thrombin by the prothrombinase enzyme complex in the coagulation cascade common pathway. The serine protease thrombin converts plasma fibrinogen to insoluble fibrin. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease [2]. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210 [3].

Typical Testing Data/Standard Curve (for reference only) of F2 elisa kit
F2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS135499. Click to request current manual
NCBI/Uniprot data below describe general gene information for F2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70,037 Da
NCBI Official Full Name
prothrombin preproprotein
NCBI Official Synonym Full Names
coagulation factor II (thrombin)
NCBI Official Symbol
NCBI Official Synonym Symbols
PT; THPH1; RPRGL2
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NCBI Protein Information
prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II
UniProt Protein Name
Prothrombin
UniProt Synonym Protein Names
Coagulation factor II
Protein Family
UniProt Gene Name
UniProt Entry Name
THRB_HUMAN
NCBI Summary for F2
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq, Jul 2008]
UniProt Comments for F2
prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.5; Protease; Secreted; Apoptosis

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region

Molecular Function: protein binding; growth factor activity; serine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: positive regulation of blood coagulation; multicellular organismal development; positive regulation of collagen biosynthetic process; proteolysis; regulation of cell shape; negative regulation of fibrinolysis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; response to wounding; acute-phase response; negative regulation of proteolysis; platelet activation; cytosolic calcium ion homeostasis; post-translational protein modification; positive regulation of cell growth; peptidyl-glutamic acid carboxylation; positive regulation of phosphoinositide 3-kinase cascade; fibrinolysis; cellular protein metabolic process; regulation of gene expression; negative regulation of astrocyte differentiation; regulation of blood coagulation; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; leukocyte migration; blood coagulation, intrinsic pathway

Disease: Thrombophilia Due To Thrombin Defect; Stroke, Ischemic; Prothrombin Deficiency, Congenital; Pregnancy Loss, Recurrent, Susceptibility To, 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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