• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

F2RL1 elisa kit :: Rat coagulation factor II (thrombin) receptor-like 1 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS924128
Unit / Price DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Typical Testing Data/Standard Curve (for reference only)
Product Name

coagulation factor II (thrombin) receptor-like 1 (F2RL1), ELISA Kit

Also Known As

Rat Proteinase-activated receptor 2, F2RL1 ELISA Kit

Product Synonym Names
Rat Proteinase-activated receptor 2 (F2RL1) ELISA kit; GPR11; PAR2; G protein-coupled receptor-11; protease-activated receptor 2; proteinase-activated receptor-2; coagulation factor II (thrombin) receptor-like 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS924128 Testing Data
Sequence Length
397
3D Structure
ModBase 3D Structure for Q63645
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat F2RL1. No significant cross-reactivity or interference between Rat F2RL1 and analogues was observed.
Assay Type
Sandwich
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 3
Immunity
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of F2RL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forF2RL1purchase
MBS924128 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the coagulation factor II (thrombin) receptor-like 1 (F2RL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F2RL1. The ELISA analytical biochemical technique of the MBS924128 kit is based on F2RL1 antibody-F2RL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F2RL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F2RL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
F2RL1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F2RL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F2RL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F2RL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F2RL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of F2RL1 elisa kit
F2RL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS924128. Click to request current manual
NCBI/Uniprot data below describe general gene information for F2RL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,441 Da
NCBI Official Full Name
proteinase-activated receptor 2
NCBI Official Synonym Full Names
coagulation factor II (thrombin) receptor-like 1
NCBI Official Symbol
F2rl1  [Similar Products]
NCBI Official Synonym Symbols
Par2; Par-2
  [Similar Products]
NCBI Protein Information
proteinase-activated receptor 2; thrombin receptor-like 1; coagulation factor II receptor-like 1; Proteinase-activated receptor-2 G protein-coupled receptor 11; Proteinase-activated receptor-2, G protein-coupled receptor 11
UniProt Protein Name
Proteinase-activated receptor 2
UniProt Synonym Protein Names
Coagulation factor II receptor-like 1; Thrombin receptor-like 1
UniProt Gene Name
F2rl1  [Similar Products]
UniProt Synonym Gene Names
Par2; PAR-2  [Similar Products]
UniProt Entry Name
PAR2_RAT
NCBI Summary for F2RL1
G-coupled protein receptor that is activated by coagulation factor II and other serine proteases; may play a role in regulation of exocrine secretion [RGD, Feb 2006]
UniProt Comments for F2RL1
F2RL1: a G-protein coupled receptor for trypsin and trypsin-like enzymes. Acts as a sensor for proteolytic enzymes generated during infection. Modulates pro-inflammatory responses, and innate and adaptive immunity. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. Activates several signaling molecules including phospholipase C (PLC), mitogen-activated protein kinase (MAPK), IKK/NFkB and Rho. Elevates intracellular calcium. Can also be transactivated by cleaved PAR1. Can signal synergistically with TLR4 and probably TLR2 in inflammatory responses and modulates TLR3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone; activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as G alpha-q, G alpha-11, G alpha-14, G alpha- 12 and G alpha-13, but probably not with G(o) alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. However, may signal through G(i) subunit alpha. Believed to be a class B receptor that internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to G alpha-q/11; the function involves dissociation of RIPK1 and TRADD from TNFR1. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds; the function is independent of G alpha-q/11 and involves promotion of cofilin dephosphoryltaion and actin filament severing. Induces redistribution of COPS5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by COPS5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as proinflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immune cells; activation enhances phagocytosis of Gram- positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses. Implicated in a number of acute and chronic inflammatory diseases such as of the joints, lungs, brain, gastrointestinal tract, periodontium, skin, and vascular systems, and in autoimmune disorders. Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. Moderate expression is detected in many organs, but none in brain or skeletal muscle. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Cellular Component: integral to plasma membrane; early endosome; plasma membrane; pseudopodium

Molecular Function: G-protein coupled receptor activity; protein binding; G-protein beta-subunit binding; G-protein alpha-subunit binding; thrombin receptor activity

Biological Process: negative regulation of JNK cascade; positive regulation of positive chemotaxis; positive regulation of cytokine secretion during immune response; protein secretion; positive regulation of JNK cascade; positive regulation of leukocyte chemotaxis; positive regulation of vasodilation; regulation of JNK cascade; negative regulation of toll-like receptor 3 signaling pathway; T cell activation during immune response; elevation of cytosolic calcium ion concentration; positive regulation of glomerular filtration; positive regulation of superoxide release; interleukin-1 beta secretion; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; defense response to virus; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of actin filament depolymerization; neutrophil activation; positive regulation of eosinophil degranulation; positive regulation of chemotaxis; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of Rho protein signal transduction; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; positive regulation of pseudopodium formation; regulation of blood coagulation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of phagocytosis, engulfment; leukocyte migration; positive regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000