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F5-Ag elisa kit :: Monkey Coagulation Factor 5 Antigen ELISA Kit

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Catalog # MBS740269
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Coagulation Factor 5 Antigen (F5-Ag), ELISA Kit

Popular Item
Full Product Name

Monkey Coagulation Factor 5 Antigen ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 614389
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of F5Ag. No significant cross-reactivity or interference between F5Ag and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between F5Ag and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This F5Ag ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey F5Ag. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of F5-Ag elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forF5-Agpurchase
MBS740269 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Coagulation Factor 5 Antigen (F5-Ag) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F5-Ag. The ELISA analytical biochemical technique of the MBS740269 kit is based on F5-Ag antibody-F5-Ag antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F5-Ag antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F5-Ag. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
F5-Ag elisa kit
Principle of the Assay: F5Ag ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F5Ag antibody and an F5Ag-HRP conjugate. The assay sample and buffer are incubated together with F5Ag-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F5Ag concentration since F5Ag from samples and F5Ag-HRP conjugate compete for the anti-F5Ag antibody binding site. Since the number of sites is limited, as more sites are occupied by F5Ag from the sample, fewer sites are left to bind F5Ag-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F5Ag concentration in each sample is interpolated from this standard curve.
Product Categories/Family for F5-Ag elisa kit

Typical Testing Data/Standard Curve (for reference only) of F5-Ag elisa kit
F5-Ag elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS740269. Click to request current manual
NCBI/Uniprot data below describe general gene information for F5-Ag. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
251,703 Da
NCBI Official Full Name
coagulation factor V
NCBI Official Synonym Full Names
coagulation factor V (proaccelerin, labile factor)
NCBI Official Symbol
NCBI Official Synonym Symbols
FVL; PCCF; THPH2; RPRGL1
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NCBI Protein Information
coagulation factor V; factor V Leiden; proaccelerin, labile factor; activated protein c cofactor; coagulation factor V jinjiang A2 domain
UniProt Protein Name
Coagulation factor V
UniProt Synonym Protein Names
Activated protein C cofactor; Proaccelerin, labile factorCleaved into the following 2 chains:Coagulation factor V heavy chain; Coagulation factor V light chain
UniProt Gene Name
UniProt Entry Name
FA5_HUMAN
NCBI Summary for F5-Ag
This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]
UniProt Comments for F5-Ag
factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family.

Protein type: Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: Golgi apparatus; extracellular space; membrane; endoplasmic reticulum; plasma membrane; extracellular region; vesicle

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; blood circulation; proteolysis; blood coagulation

Disease: Thrombophilia Due To Activated Protein C Resistance; Budd-chiari Syndrome; Pregnancy Loss, Recurrent, Susceptibility To, 1; Stroke, Ischemic; Factor V Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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