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FABP2 elisa kit :: Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit

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Catalog # MBS451944
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Fatty Acid Binding Protein 2, Intestinal (FABP2), ELISA Kit

Popular Item
Full Product Name

Mouse Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit

Product Synonym Names
FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P55050
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of FABP2. No significant cross-reactivity or interference between FABP2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.063ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FABP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FABP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FABP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FABP2purchase
MBS451944 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fatty Acid Binding Protein 2, Intestinal (FABP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FABP2. The ELISA analytical biochemical technique of the MBS451944 kit is based on FABP2 antibody-FABP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FABP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FABP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FABP2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FABP2 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FABP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FABP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of FABP2 elisa kit
FABP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS451944. Click to request current manual
NCBI/Uniprot data below describe general gene information for FABP2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
15,126 Da
NCBI Official Full Name
fatty acid-binding protein, intestinal
NCBI Official Synonym Full Names
fatty acid binding protein 2, intestinal
NCBI Official Symbol
Fabp2  [Similar Products]
NCBI Official Synonym Symbols
Fabpi; I-FABP
  [Similar Products]
NCBI Protein Information
fatty acid-binding protein, intestinal
UniProt Protein Name
Fatty acid-binding protein, intestinal
UniProt Synonym Protein Names
Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP
UniProt Gene Name
Fabp2  [Similar Products]
UniProt Synonym Gene Names
Fabpi; I-FABP  [Similar Products]
NCBI Summary for FABP2
The protein encoded by this gene is part of the fatty acid binding protein family (FABP). FABPs are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands and participate in fatty acid uptake, transport, and metabolism. This protein functions within enterocytes, possibly to sense lipids as part of energy homeostasis. In humans polymorphisms are associated with increased fat oxidation and insulin resistance. In mice deficiency of this gene alters body weight in a gender-specific manner and causes hyperinsulinemia. [provided by RefSeq, Jan 2013]
UniProt Comments for FABP2
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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