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FABP4 elisa kit :: Mouse fatty acid binding protein 4, adipocyte ELISA Kit

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Catalog # MBS925921
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
FABP4 elisa kit
Product Name

fatty acid binding protein 4, adipocyte (FABP4), ELISA Kit

Popular Item
Also Known As

Mouse Fatty acid-binding protein, adipocyte, FABP4 ELISA Kit

Product Synonym Names
Mouse Fatty acid-binding protein; adipocyte (FABP4) ELISA kit; A-FABP; aP2; ; fatty acid binding protein 4; adipocyte
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
132
3D Structure
ModBase 3D Structure for P04117
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse FABP4. No significant cross-reactivity or interference between mouse FABP4 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FABP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFABP4purchase
MBS925921 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fatty acid binding protein 4, adipocyte (FABP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FABP4. The ELISA analytical biochemical technique of the MBS925921 kit is based on FABP4 antibody-FABP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FABP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FABP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FABP4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FABP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FABP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS925921. Click to request current manual
NCBI/Uniprot data below describe general gene information for FABP4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
14,650 Da
NCBI Official Full Name
fatty acid-binding protein, adipocyte
NCBI Official Synonym Full Names
fatty acid binding protein 4, adipocyte
NCBI Official Symbol
Fabp4  [Similar Products]
NCBI Official Synonym Symbols
Ap2; Lbpl; 422/aP2; ALBP/Ap2
  [Similar Products]
NCBI Protein Information
fatty acid-binding protein, adipocyte; P15; ALBP; AFABP; A-FABP; protein 422; P2 adipocyte protein; adipocyte protein aP2; myelin P2 protein homolog; 3T3-L1 lipid-binding protein; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein
UniProt Protein Name
Fatty acid-binding protein, adipocyte
UniProt Synonym Protein Names
3T3-L1 lipid-binding protein; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422
UniProt Gene Name
Fabp4  [Similar Products]
UniProt Synonym Gene Names
Ap2; ALBP; A-FABP; AFABP  [Similar Products]
UniProt Entry Name
FABP4_MOUSE
UniProt Comments for FABP4
FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: transporter activity; lipid binding

Biological Process: cholesterol homeostasis; transport; positive regulation of cell proliferation; white fat cell differentiation; brown fat cell differentiation; cytokine production; negative regulation of protein kinase activity; fatty acid metabolic process; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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