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FasL recombinant protein

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Catalog # MBS536558
Unit / Price
  0.01 mg  /  $310 +1 FREE 8GB USB
FasL recombinant protein
Product Name

FasL, Recombinant Protein

Popular Item
Also Known As

FasL protein

Product Synonym Names
Apo I Ligand protein; CD95L protein; TNFSF6 protein; soluble Fas Ligand protein; APTL protein; sFASL protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
281
Sequence
Region of FasL protein correspond ing to amino acids HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL.
OMIM
134638
3D Structure
ModBase 3D Structure for P48023
Host
Human
Purity/Purification
> 98% by SDS-PAGE gel and HPLC Analyses.
Form/Format
Sterile filtered through a 0.2 micron filter. Lyophilized from 1 x PBS, pH 7.2. Reconsitute in water to a concentration of 0.1-1.0 mg/ml
Concentration
10ug per vial (lot specific)
Protein Type
Recombinant
Biological Significance
Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.
Bioactivity
Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is approximately 10.0 ng/ml, corresponding to a specific activity of approximately 1 x 10^5 units/mg.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Expression System
CHO cells
Biohazard
Use standard laboratory precautions when handling this material.
Preparation and Storage
Ships ambient or refrigerated. Upon receipt store at 4 degree C short term, -20 degree C long term. After reconsituted, the solution can be stored at 4 degree C for up to 1 week. Working aliquots stored with a carrier protein are stable for at least 12 months at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of FasL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FasL recombinant protein
Purified soluble Human FasL protein
Product Categories/Family for FasL recombinant protein
Applications Tested/Suitable for FasL recombinant protein
User optimized
Application Notes for FasL recombinant protein
Specific activity is > 2 x 10^5 units/mg
NCBI/Uniprot data below describe general gene information for FasL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17.6 kDa
NCBI Official Full Name
Fas ligand
NCBI Official Synonym Full Names
Fas ligand (TNF superfamily, member 6)
NCBI Official Symbol
FASLG  [Similar Products]
NCBI Official Synonym Symbols
APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
  [Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 6
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 6
UniProt Synonym Protein Names
Apoptosis antigen ligand; APTL
UniProt Gene Name
FASLG  [Similar Products]
UniProt Synonym Gene Names
APT1LG1; CD95L; FASL; TNFSF6; APTL; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA  [Similar Products]
UniProt Entry Name
TNFL6_HUMAN
NCBI Summary for FasL
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
UniProt Comments for FasL
FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: lysosomal lumen; extracellular space; integral to plasma membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; caveola; nucleus; external side of plasma membrane

Molecular Function: protein binding; death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; signal transduction; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; induction of apoptosis via death domain receptors; cell-cell signaling; positive regulation of neuron apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; retinal cell programmed cell death; immune response

Disease: Lung Cancer; Autoimmune Lymphoproliferative Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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