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FceRII/CD23 elisa kit :: Bovine Receptor II for the Fc region of immunoglobulin E ELISA Kit

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Catalog # MBS745231
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Receptor II for the Fc region of immunoglobulin E (FceRII/CD23), ELISA Kit

Also Known As

Bovine Receptor II for the Fc region of immunoglobulin E ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
151445
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of IgE-FcR 2. No significant cross-reactivity or interference between IgE-FcR 2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IgE-FcR 2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This IgE-FcR 2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine IgE-FcR 2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FceRII/CD23 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFceRII/CD23purchase
MBS745231 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Receptor II for the Fc region of immunoglobulin E (FceRII/CD23) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FceRII/CD23. The ELISA analytical biochemical technique of the MBS745231 kit is based on FceRII/CD23 antibody-FceRII/CD23 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FceRII/CD23 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FceRII/CD23. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FceRII/CD23 elisa kit
Principle of the assay: IgE-FcR 2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IgE-FcR 2 antibody and an IgE-FcR 2-HRP conjugate. The assay sample and buffer are incubated together with IgE-FcR 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgE-FcR 2 concentration since IgE-FcR 2 from samples and IgE-FcR 2-HRP conjugate compete for the anti-IgE-FcR 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IgE-FcR 2 from the sample, fewer sites are left to bind IgE-FcR 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgE-FcR 2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FceRII/CD23 elisa kit

Typical Testing Data/Standard Curve (for reference only) of FceRII/CD23 elisa kit
FceRII/CD23 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS745231. Click to request current manual
NCBI/Uniprot data below describe general gene information for FceRII/CD23. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
36,469 Da
NCBI Official Full Name
low affinity immunoglobulin epsilon Fc receptor isoform b
NCBI Official Synonym Full Names
Fc fragment of IgE, low affinity II, receptor for (CD23)
NCBI Official Symbol
FCER2  [Similar Products]
NCBI Official Synonym Symbols
CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
  [Similar Products]
NCBI Protein Information
low affinity immunoglobulin epsilon Fc receptor; CD23 antigen; fc-epsilon-RII; lymphocyte IgE receptor; immunoglobulin E-binding factor; C-type lectin domain family 4, member J
UniProt Protein Name
Low affinity immunoglobulin epsilon Fc receptor
UniProt Synonym Protein Names
BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD_antigen: CD23Cleaved into the following 2 chains:Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form
UniProt Gene Name
FCER2  [Similar Products]
UniProt Synonym Gene Names
CD23A; CLEC4J; FCE2; IGEBF  [Similar Products]
UniProt Entry Name
FCER2_HUMAN
NCBI Summary for FceRII/CD23
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
UniProt Comments for FceRII/CD23
Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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